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Antifungal Agents

Antifungal Agents

Amphotericin B is a broad-spectrum antifungal agent that exploits structural differences between fungal and mammalian cell membranes. Its amphipathic structure—featuring a hydrophobic polyene-lactone ring and a hydrophilic region containing mycosamine and carboxylic acid groups—enables selective binding to ergosterol, a sterol predominantly found in fungal plasma membranes. This selective interaction underlies the drug’s antifungal activity, although weak binding to cholesterol contributes to...

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Updated: Jul 6, 2026

Automation of Bio-Atomic Force Microscope Measurements on Hundreds of C. albicans Cells

Automation of Bio-Atomic Force Microscope Measurements on Hundreds of C. albicans Cells

Published on: April 2, 2021

Caspofungin disk diffusion breakpoints and quality control.

Steven D Brown¹, Maria M Traczewski

¹The Clinical Microbiology Institute, 9725 SW Commerce Circle, Wilsonville, Oregon 97070, USA. SBrown@clinmicroinst.com

Journal of Clinical Microbiology

|April 11, 2008

Summary

This summary is machine-generated.

New disk diffusion breakpoints for caspofungin, an antifungal drug, are proposed. This method accurately predicts susceptibility in Candida species, aiding clinical decisions for fungal infections.

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Published on: October 21, 2010

Area of Science:

Antimicrobial Susceptibility Testing
Clinical Mycology
Pharmaceutical Sciences

Background:

Accurate susceptibility testing is crucial for effective antifungal therapy.
Caspofungin is a key echinocandin antifungal agent.
Disk diffusion offers a simpler alternative to broth microdilution for susceptibility testing.

Purpose of the Study:

To establish interpretive disk diffusion breakpoints for caspofungin against Candida species.
To evaluate the performance of caspofungin disk diffusion compared to reference broth microdilution methods.
To propose quality control (QC) ranges for disk diffusion testing of caspofungin.

Main Methods:

Evaluated 762 Candida spp. isolates from clinical trials.
Compared zone diameters from 5-microg caspofungin disks (two manufacturers) against standardized broth microdilution MICs.
Conducted an eight-laboratory disk diffusion quality control study.

Main Results:

Proposed disk diffusion breakpoints of >=11 mm for susceptible caspofungin.
Achieved error rates <=0.3% compared to MIC testing.
Proposed QC ranges for four CLSI-recommended QC strains.

Conclusions:

Disk diffusion provides a reliable and accurate method for predicting caspofungin susceptibility in Candida.
The proposed breakpoints and QC ranges support the clinical utility of this method.
This facilitates simpler and faster antifungal susceptibility testing in clinical laboratories.