Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Size and Structure of Viral Genomes01:26

Size and Structure of Viral Genomes

Viral genomes exhibit remarkable diversity in size, structure, and composition, influencing their replication strategies and interactions with host cells. These genomes consist of either DNA or RNA and may be linear or circular. Additionally, they can be single-stranded or double-stranded, with each configuration affecting how the virus propagates within a host. RNA viruses, for instance, generally have smaller genomes than DNA viruses, a factor that contributes to their high mutation rates and...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

mRNA Delivery by Lipoamino Fatty Acid-Peptide Polyplexes in Different Lung Cell Models and Lungs.

Polymers·2026
Same author

Dissecting mechanisms of ligand binding and conformational changes in the glutamine-binding protein.

eLife·2026
Same author

New insights into the intrinsic tryptophan fluorescence emission spectra of IgG antibodies and the impact of protein unfolding and aggregation.

European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V·2026
Same author

mtHsp70 chaperone converts mitochondrial proteostasis stress into impaired protein import.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

An analysis of cost-saving in the German statutory health insurance system due to the introduction of epoetin alfa biosimilars in Germany.

Frontiers in public health·2026
Same author

Mapping the Functional Architecture of Influenza A Virus-Induced Phase Separation through m<sup>6</sup>A Proximity Labeling.

ACS nano·2025

Related Experiment Video

Updated: Jul 6, 2026

Single-cell Quantitation of mRNA and Surface Protein Expression in Simian Immunodeficiency Virus-infected CD4+ T Cells Isolated from Rhesus macaques
13:13

Single-cell Quantitation of mRNA and Surface Protein Expression in Simian Immunodeficiency Virus-infected CD4+ T Cells Isolated from Rhesus macaques

Published on: September 25, 2018

HIV-1-cellular interactions analyzed by single virus tracing.

Thomas Endress1, Marko Lampe, John A G Briggs

  • 1Physical Chemistry, Department of Chemistry and Biochemistry, Ludwig-Maximilians-Universität München, Butenandtstr. 11, Haus E, 81377, Munich, Germany.

European Biophysics Journal : EBJ
|April 11, 2008
PubMed
Summary

Single virus tracing reveals how human immunodeficiency virus type 1 (HIV-1) interacts with cell surfaces. Particle immobilization depends on heparan sulfate proteoglycans (HSPG) concentration, influencing viral entry pathways.

More Related Videos

Single-Cell Characterization of Calcium Influx and HIV-1 Infection using a Multiparameter Optofluidic Platform
07:15

Single-Cell Characterization of Calcium Influx and HIV-1 Infection using a Multiparameter Optofluidic Platform

Published on: May 18, 2021

Imaging of HIV-1 Envelope-induced Virological Synapse and Signaling on Synthetic Lipid Bilayers
11:45

Imaging of HIV-1 Envelope-induced Virological Synapse and Signaling on Synthetic Lipid Bilayers

Published on: March 8, 2012

Related Experiment Videos

Last Updated: Jul 6, 2026

Single-cell Quantitation of mRNA and Surface Protein Expression in Simian Immunodeficiency Virus-infected CD4+ T Cells Isolated from Rhesus macaques
13:13

Single-cell Quantitation of mRNA and Surface Protein Expression in Simian Immunodeficiency Virus-infected CD4+ T Cells Isolated from Rhesus macaques

Published on: September 25, 2018

Single-Cell Characterization of Calcium Influx and HIV-1 Infection using a Multiparameter Optofluidic Platform
07:15

Single-Cell Characterization of Calcium Influx and HIV-1 Infection using a Multiparameter Optofluidic Platform

Published on: May 18, 2021

Imaging of HIV-1 Envelope-induced Virological Synapse and Signaling on Synthetic Lipid Bilayers
11:45

Imaging of HIV-1 Envelope-induced Virological Synapse and Signaling on Synthetic Lipid Bilayers

Published on: March 8, 2012

Area of Science:

  • Virology
  • Cell Biology
  • Biophysics

Background:

  • Single virus tracing (SVT) is a powerful technique for observing viral entry into living cells.
  • Understanding the initial interactions between viruses and the cell membrane is crucial for deciphering infection mechanisms.

Purpose of the Study:

  • To investigate the interaction dynamics between human immunodeficiency virus type 1 (HIV-1) and the plasma membrane of living cells using SVT.
  • To determine factors influencing HIV-1 particle attachment and contact duration on the cell surface.

Main Methods:

  • Utilized fluorescently labeled virus-like particles (VLPs) for SVT.
  • Analyzed VLP trajectories to determine particle size and diffusion characteristics.
  • Quantified VLP immobilization and contact durations on various cell lines.

Main Results:

  • Prepared VLPs were homogeneous with a hydrodynamic radius of 86 +/- 5 nm, consistent with HIV-1.
  • VLPs either adhered to the cell surface or rapidly dissociated.
  • VLP immobilization fraction correlated positively with surface heparan sulfate proteoglycans (HSPG) concentration.
  • Non-immobilized VLPs made an average of 1.5 contacts before diffusing away.
  • Contact durations ranged from 20 to 50 ms, varying by cell type.

Conclusions:

  • HIV-1 interaction with the plasma membrane is a dynamic process involving transient contacts and immobilization.
  • Heparan sulfate proteoglycans (HSPG) play a significant role in mediating HIV-1 attachment to cell surfaces.
  • SVT provides valuable insights into the initial steps of viral entry and host cell interactions.