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Updated: Jul 6, 2026
Assessment of Sarcoplasmic Reticulum Calcium Reserve and Intracellular Diastolic Calcium Removal in Isolated Ventricular Cardiomyocytes
Published on: September 18, 2017
[The effects of heroin on intracellular free Ca2+ of rat myocardium]
Xiao-Shan Liu1, Jing-Dong Wu, Zi-Rui Hao
1Department of Forensic Medicine, Medical College of Sun Yat-sen University, Guangzhou, China. lxsa200@yahoo.com.cn
Objective:
To observe the effects of heroin on intracellular free Ca2+ in rat myocardium.
Methods:
The effects of heroin on intracellular free Ca2+ were observed in cultured neonatal rat myocardium by measuring intracellular free Ca2+ concentration using calcium fluorescent probe Flou-3/AM and laser scanning confocal microscope.
Results:
Different doses and concentrations of heroin appeared to have different effects on intracellular free Ca2+ concentrations, with a dosage dependent short linear increase in the fluorescence intensity (i.e., Ca2+ concentration) leading to [Ca2+]i peak.
Conclusion:
Heroin could affect concentrations of [Ca2+]i in myocardium and its dosage related effect needs further investigation.
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