Related Experiment Video
Updated: Jul 6, 2026
08:01
Measurement of Insulin- and Contraction-Stimulated Glucose Uptake in Isolated and Incubated Mature Skeletal Muscle from Mice
Published on: May 16, 2021
[AMPK as a cellular energy sensor and its function in the organism]
Natalia Miranda1, Armando R Tovar, Berenice Palacios
1Departamento de Fisiología de la Nutrición, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Tlalpan, México.
Summary
Adenine monophosphate (AMP) activated protein kinase (AMPK) acts as a cellular energy sensor, regulating metabolic processes. This review explores AMPK
Area of Science:
- Biochemistry and Molecular Biology
- Cellular Metabolism
- Physiology
Context:
- Adenine monophosphate (AMP) activated protein kinase (AMPK) is a crucial cellular energy sensor.
- It responds to changes in the AMP/ATP ratio, indicating cellular energy status.
- AMPK plays a vital role in regulating overall energy balance and caloric intake.
Purpose:
- To provide a comprehensive overview of AMPK's structure and activation mechanisms.
- To elucidate the diverse functions of AMPK in cellular and organismal metabolism.
- To highlight AMPK's role as a potential therapeutic target for metabolic diseases.
Summary:
- AMPK, a heterotrimeric complex, is activated by LKB1 kinase and modulates anabolic and catabolic pathways to maintain ATP levels.
- It influences gene expression related to lipogenesis and mitochondrial biogenesis.
- AMPK is present in various organs, including the liver, muscle, heart, and hypothalamus, impacting appetite and metabolic functions like glycolysis, glucose uptake, and lipid metabolism.
Impact:
- Understanding AMPK's function is key to developing treatments for obesity, type 2 diabetes, and hepatic steatosis.
- AMPK's role in energy homeostasis offers insights into metabolic disease pathogenesis.
- This review consolidates current knowledge on AMPK, facilitating further research in metabolic regulation.
More Related Videos
08:04Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method
Published on: October 23, 2018
08:03Live-Cell Förster Resonance Energy Transfer Imaging of Metabolically Regulated Akt Activation Dynamics in HepG2 Cells
Published on: May 23, 2025
Related Concept Videos
01:25cAMP-dependent Protein Kinase Pathways
Cyclic Adenosine Monophosphate (cAMP) is an essential second messenger that activates protein kinase A (PKA) and regulates various biological processes. A single epinephrine molecule binds to GPCR and activates several heterotrimeric G proteins, each stimulating multiple adenylyl cyclase, amplifying the signal, and synthesizing large numbers of cAMP molecules. Small changes in cAMP concentration affect PKA activity. The binding of four cAMP molecules induces a conformational change in PKA,...
01:22PI3K/mTOR/AKT Signaling Pathway
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a rapamycin-insensitive companion...
02:54Protein Kinases and Phosphatases
Proteins undergo chemical modifications that trigger changes in the charge, structure, and conformation of the proteins. Phosphorylation, acetylation, glycosylation, nitrosylation, ubiquitination, lipidation, methylation, and proteolysis are various protein modifications that regulate protein activity. Such modifications are usually enzyme-driven.
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
01:30Introduction to Metabolism
Metabolism encompasses all biochemical reactions in a living organism, facilitating both the breakdown and synthesis of biomolecules. These metabolic processes are categorized into catabolic and anabolic pathways, which operate in a coordinated manner to ensure energy balance and cellular function.Catabolic Pathways and Energy ReleaseCatabolic pathways involve the breakdown of complex macromolecules such as carbohydrates, lipids, and proteins into smaller structures like monosaccharides, fatty...
01:28Global Regulatory Systems
Global regulatory systems in bacteria enable rapid and coordinated responses to environmental changes by integrating sensory inputs with gene expression, ensuring efficient adaptation to fluctuating conditions. Key global regulatory mechanisms include regulons, two-component systems, sigma factors, and secondary messengers.Regulons and Global RegulatorsA regulon is a collection of genes and operons controlled by a common global regulator. These regulators enable bacteria to prioritize resource...
01:19Regulation of Metabolism
Cellular needs and conditions vary from cell to cell and change within individual cells over time. For example, the required enzymes and energetic demands of stomach cells are different from those of fat storage cells, skin cells, blood cells, and nerve cells. Furthermore, a digestive cell works much harder to process and break down nutrients during the time that closely follows a meal compared with many hours after a meal. As these cellular demands and conditions vary, so do the amounts and...