Related Experiment Video
Updated: Aug 14, 2026

Colony Forming Cell (CFC) Assay for Human Hematopoietic Cells
Published on: December 18, 2010
Mitogenic response to colony-stimulating factor 1
1Howard Hughes Medical Institute, Department of Tumor Cell Biology, St Jude Children's Research Hospital, Memphis, TN 38105.
Abstract:
The proliferative effects of colony-stimulating factor 1 (CSF-1) on cells of the mononuclear phagocyte lineage are mediated by the CSF-1 receptor tyrosine kinase, which triggers intracellular signal transduction through multiple second messenger pathways. CSF-1 is required throughout G1 to stimulate both immediate- and delayed-early responses that ensure entry of macrophages into S phase. Because CSF-1 induces the expression of different sets of cellular genes during early and late G1, products of the immediate-early response might modulate the effects of ensuing receptor signals to facilitate G1 progression. Targets of the delayed-early response include novel cyclin genes that may play a role in S phase commitment.
More Related Videos
11:55Induction of Alloantigen-specific Anergy in Human Peripheral Blood Mononuclear Cells by Alloantigen Stimulation with Co-stimulatory Signal Blockade
Published on: March 14, 2011
06:20Assessment of the Immunomodulatory Properties of Human Mesenchymal Stem Cells (MSCs)
Published on: December 24, 2015
Related Concept Videos
Mitogens and the Cell Cycle
Differentiation of Common Myeloid Progenitor Cells
Regulation of Hematopoietic Stem Cells
TGF - β Signaling Pathway
Mitogens and the Cell Cycle
Target Cell Response to Hormones
Notably, the cellular response can be regulated by altering the number of receptors expressed in the cell. For example, prolonged exposure to elevated hormone levels results in a gradual decline or down-regulation in the number of receptors for that specific hormone on the cell surface. Conversely, in response to low hormone levels, cells may use up-regulation, producing an...