Amperometric biosensor based on glucose dehydrogenase and plasma-polymerized thin films
Atsunori Hiratsuka1, Kohta Fujisawa, Hitoshi Muguruma
1Research Center of Advanced Bionics, National Institute of Advanced Industrial Science and Technology (AIST), c/o Katayanagi Advanced Research Laboratories, Tokyo University of Technology, Tokyo, Japan.
Summary
A novel amperometric biosensor utilizes glucose dehydrogenase (GDH) and plasma-polymerized thin films (PPF) for efficient glucose detection. This dry-chemistry method offers a scalable alternative for bioelectronic device manufacturing.
Area of Science:
- Electrochemistry
- Materials Science
- Biotechnology
Background:
- Amperometric biosensors are crucial for glucose monitoring.
- Traditional fabrication methods face limitations in mass production.
- Plasma-polymerized thin films (PPF) offer unique interfacial properties.
Purpose of the Study:
- To develop a novel amperometric biosensor design for glucose detection.
- To integrate NAD(P)-dependent glucose dehydrogenase (GDH) with PPF.
- To explore a dry-chemistry fabrication process for bioelectronic devices.
Main Methods:
- A sandwich structure of PPF/GDH/PPF was fabricated on a gold electrode.
- The lower PPF layer served as a hydrophilic enzyme-electrode interface.
- The upper PPF layer acted as an overcoating for immobilized GDH.
Main Results:
- The biosensor demonstrated optimal performance for glucose concentrations between 2.5-26 mM.
- Achieved a sensitivity of 320 nA mM(-1) cm(-2) at +0.6 V applied potential.
- Exhibited a high correlation coefficient of 0.990, indicating excellent linearity.
Conclusions:
- The novel PPF-based amperometric biosensor offers a promising platform for glucose sensing.
- The dry-chemistry fabrication method is compatible with high-throughput production.
- This approach has significant potential for manufacturing advanced bioelectronic devices.
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