Nucleotide sequence of trpE, anthranilate synthase I gene, of Bacillus caldotenax

trpE gene of Bacillus caldotenax was cloned by complementation of an Escherichia coli tryptophan auxotroph. The trpE encoding a 508 amino acid polypeptide is followed by trpD. S1-nuclease mapping of a trpE in vivo transcript suggested that a promoter preceding the trpE was utilized by E. coli. The deduced amino acid sequence of anthranilate synthase I (ASI) was compared with that of five other bacterials ASIs.