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Updated: Jul 6, 2026
Fluorescence Recovery after Merging a Droplet to Measure the Two-dimensional Diffusion of a Phospholipid Monolayer
Published on: October 15, 2015
Study of the interaction of beta-cyclodextrin with phospholipid monolayers by surface pressure measurements and
Christine Grauby-Heywang1, Jean-Marie Turlet
1Centre de Physique Moléculaire Optique et Hertzienne, UMR CNRS 5798 Université Bordeaux 1, 351 Cours de la Libération, 33405 Talence cedex, France. ch.heywang@cpmoh.u-bordeaux1.fr
Abstract:
The interaction of beta-cyclodextrin (beta-CD) with different lipids has been studied, using Langmuir monolayers kept at constant surface pressure or constant spreading surface. Results show that beta-CD, injected beneath the monolayer, is able to desorb unsaturated palmitoyloleoylphosphatidylcholine (POPC) and sphingomyelin (SM) under specific experimental conditions. In this last case, SM monolayers, labeled with the fluorescent NBD-PC probe, were also observed by fluorescence microscopy, before and after beta-CD injection. Images show that SM monolayers are more homogeneous after beta-CD injection, because of the lipid desorption. At last, it seems that lipid desorption occurs only in a restricted surface pressure range, depending on the lipid.
