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C-myb proto-oncogene: evidence for intermolecular recombination of coding sequences
M Vellard1, J Soret, E Viegas-Pequignot
1Institut Curie, Centre Universitaire, Orsay, France.
Oncogene
|April 1, 1991
Summary
Researchers discovered a new chicken c-myb exon in thymus cells, suggesting recombination is key for proto-oncogene expression. This finding has implications for understanding gene regulation in both chickens and humans.
Area of Science:
- Genetics
- Molecular Biology
- Immunology
Background:
- The c-myb proto-oncogene plays a crucial role in cell proliferation and differentiation.
- Differential expression of c-myb is observed across various cell types, including hematopoietic and thymic cells.
Purpose of the Study:
- To characterize a novel thymus-specific exon of the chicken c-myb gene.
- To investigate the mechanism underlying the tissue-specific expression of c-myb.
- To explore the evolutionary conservation and localization of c-myb sequences in humans.
Main Methods:
- In situ hybridization to determine exon localization.
- Comparative genomic analysis of chicken and human c-myb loci.
- DNA sequencing and Polymerase Chain Reaction (PCR) analyses of chicken thymic c-myb mRNA.
Main Results:
- A novel chicken c-myb exon, specifically expressed in thymic cells, was identified.
- This thymus-specific exon is located on a small chromosome, separate from the main c-myb locus on chromosome 3.
- Thymus-specific sequences are conserved in human DNA and map to chromosome 17q25, distinct from the human c-myb locus on 6q22-23.
- A repeated decameric sequence appears to be involved in the recombination process driving tissue-specific expression.
Conclusions:
- Intermolecular recombination is essential for the tissue-specific expression of the chicken c-myb proto-oncogene.
- The findings suggest a complex regulatory mechanism involving genomic rearrangement for c-myb gene expression.
- Conserved sequences and distinct chromosomal localizations highlight evolutionary aspects of c-myb regulation.
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