Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Gonadal and Placental Hormones01:24

Gonadal and Placental Hormones

The gonads, namely the testes in males and the ovaries in females, are pivotal in producing gonadal hormones that orchestrate the intricate processes of sexual development and reproduction.
In males, testosterone is the primary gonadal androgen. It plays a central role in the maturation of male reproductive organs — the penis and testes. Additionally, testosterone is instrumental in the development of secondary sexual characteristics — a deep voice as well as facial and pubic hair growth — and...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Bridge percolation: electrical connectivity of discontinued conducting slabs by metallic nanowires.

Nanoscale·2024
Same author

Understanding the mechanisms leading to failure in metallic nanowire-based transparent heaters, and solution for stability enhancement.

Nanotechnology·2016
Same author

Optimization of silver nanowire-based transparent electrodes: effects of density, size and thermal annealing.

Nanoscale·2015
Same author

Variation in transplacental transfer of tyrosine kinase inhibitors in the human perfused cotyledon model.

Annals of oncology : official journal of the European Society for Medical Oncology·2015
Same author

Specific detection of type II human chorionic gonadotropin beta subunit produced by trophoblastic and neoplastic cells.

Clinica chimica acta; international journal of clinical chemistry·2015
Same author

Metallic nanowire networks: effects of thermal annealing on electrical resistance.

Nanoscale·2014

Related Experiment Video

Updated: Jul 6, 2026

Microsatellite DNA Genotyping and Flow Cytometry Ploidy Analyses of Formalin-fixed Paraffin-embedded Hydatidiform Molar Tissues
11:54

Microsatellite DNA Genotyping and Flow Cytometry Ploidy Analyses of Formalin-fixed Paraffin-embedded Hydatidiform Molar Tissues

Published on: October 20, 2019

Human chorionic gonadotropin Molecular forms, detection, and clinical implications.

J M Bidart1, D Bellet

  • 1Department of Molecular Immunology, Institut Gustave-Roussy, Villejuif, France.

Trends in Endocrinology and Metabolism: TEM
|November 1, 1993
PubMed
Summary

This study details the various molecular forms of human chorionic gonadotropin (hCG), including intact hCG, free subunits, and fragments. Accurate measurement of these hCG forms is crucial for diagnosing and monitoring various cancers and diseases.

More Related Videos

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
09:06

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

Related Experiment Videos

Last Updated: Jul 6, 2026

Microsatellite DNA Genotyping and Flow Cytometry Ploidy Analyses of Formalin-fixed Paraffin-embedded Hydatidiform Molar Tissues
11:54

Microsatellite DNA Genotyping and Flow Cytometry Ploidy Analyses of Formalin-fixed Paraffin-embedded Hydatidiform Molar Tissues

Published on: October 20, 2019

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
09:06

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

Area of Science:

  • Biochemistry
  • Oncology
  • Reproductive Endocrinology

Background:

  • Human chorionic gonadotropin (hCG) exists in multiple molecular forms in biological fluids.
  • These forms include intact hCG, free alpha and beta subunits (hCGalpha, hCGbeta), nicked hCG, and the beta-core fragment (hCGbetacf).
  • Immunological assays are commonly used for hCG determination, but kit discrepancies pose clinical challenges.

Purpose of the Study:

  • To elucidate the diverse molecular forms of human chorionic gonadotropin (hCG) found in biological fluids.
  • To highlight the clinical significance of measuring specific hCG forms for disease diagnosis and management.
  • To address the challenges associated with current immunoassay methods for hCG and its related molecules.

Main Methods:

  • Identification and characterization of various hCG molecular forms.
  • Review of immunological procedures for hCG determination.
  • Analysis of discrepancies among different commercial immunoassay kits.

Main Results:

  • Multiple hCG variants identified: intact hCG, free hCGalpha, free hCGbeta, nicked hCG, and hCGbetacf.
  • Specific measurement of intact hCG and free beta subunit (hCGbeta) is vital for diagnosing trophoblastic diseases and testicular cancers.
  • Free hCGbeta alone is the target analyte for detecting nongonadal and nonplacental tumors.

Conclusions:

  • Understanding the heterogeneity of hCG molecular forms is essential for accurate clinical interpretation.
  • Standardization of immunoassay kits is needed to overcome discrepancies in hCG measurements.
  • Targeted measurement of specific hCG forms offers valuable diagnostic and prognostic information in oncology and reproductive medicine.