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Simple preparation of baicalin from Scutellariae Radix
Emika Ohkoshi1, Tomomi Nagashima, Hiroyasu Sato
1The School of Pharmaceutical Sciences, Ohu University 31-1, Misumidou Tomita-Machi, Kooriyama-shi, Fukushima 963-8611, Japan. e-ohkoshi@pha.ohu-u.ac.jp
Abstract:
A simple, rapid and reproducible method was developed to isolate high-purity baicalin from Scutellariae Radix, the dried roots of Scutellaria baicalensis Georgi. The method involves partition/recrystallization steps without repeated column chromatography or special instruments. Isolated baicalin was characterized by comparisons of TLC, HPLC, IR, MS, and NMR data with an authentic sample. Moreover, beta-glucuronidase hydrolysis of baicalin sequentially yielded glucuronic acid and baicalein as confirmed by co-TLC with authentic samples. The purity of baicalin was more than 97% with yield ca. 8.7% (w/w). The method presented here appears suitable for commercial application.
