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Updated: Jul 6, 2026

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers
Published on: May 5, 2016
Novel wavelength-resolved fluorescence detection for a high-throughput capillary electrophoresis system under a
Shi-Wei Lin1, Guan-Liang Chang, Che-Hsin Lin
1Institute of Biomedical Engineering, National Cheng Kung University, Tainan 70428, Taiwan.
Abstract:
This paper presents a novel method regarding a wavelength-resolved fluorescence detection scheme for high-throughput analysis of bio-samples in a micro-CE chip. Instead of using the conventional laser-induced fluorescence (LIF) microscope equipped with delicate spatial filters and complex control systems, this study adopts a hollow cone illumination generated using a dark-field condenser for exciting fluorescence in the microchannel and an ultraviolet-visible-near-infrared (UV-Vis-NIR) spectrometer for detecting the emission signals. Experimental results show that the proposed system is feasible for simultaneously detecting a mixed sample composed of Atto 610, Rhodamine B and fluorescein isothiocyanate (FITC) fluorescent dyes in a single test run. Furthermore, a mixed bio-sample composed of two mixed 16-mer single-stranded DNAs labeled with Cy3 and FITC fluorescent dyes is also successfully detected with the proposed system. The measured limit of detection (LOD) for detecting FITC of the proposed system can be as low as 5.4x10(-6)M (S/N=3). This proposed detection method has shown its potential on RNA identification and DNA sequencing applications.
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