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Synthesis and proteomic activity evaluation of a new isotope-coded affinity tagging (ICAT) reagent

Annalisa Guaragna1, Angela Amoresano, Vittoria Pinto

  • 1Dipartimento di Chimica Organica e Biochimica Università di Napoli Federico II Via Cinthia, 4 I-80126 Napoli, Italy. guaragna@unina.it

Bioconjugate Chemistry
|April 15, 2008
PubMed

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Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...