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Related Experiment Video

Updated: Jul 6, 2026

Medium-throughput Screening Assays for Assessment of Effects on Ca2+-Signaling and Acrosome Reaction in Human Sperm

Medium-throughput Screening Assays for Assessment of Effects on Ca²⁺-Signaling and Acrosome Reaction in Human Sperm

Published on: March 1, 2019

Fas receptor is not present on ejaculated human sperm.

S Perticarari¹, G Ricci, R Boscolo

¹Clinical Analysis Unit, Department of Laboratory Medicine, Institute of Child Health IRCCS Burlo Garofolo, Via dell'Istria 65/1, 34137 Trieste, Italy.

Human Reproduction (Oxford, England)

|April 15, 2008

Summary

Fas (CD95) is not detected on human ejaculated sperm, despite its role in germ cell apoptosis. This study found no correlation between Fas on sperm and apoptotic markers, suggesting Fas is not a reliable indicator of sperm apoptosis.

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Last Updated: Jul 6, 2026

Medium-throughput Screening Assays for Assessment of Effects on Ca2+-Signaling and Acrosome Reaction in Human Sperm

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Published on: March 1, 2019

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Fluorimetric Techniques for the Assessment of Sperm Membranes

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Published on: November 28, 2018

Area of Science:

Reproductive Biology
Immunology
Cell Biology

Background:

Apoptosis regulates male germ cell numbers.
Fas expression is observed in apoptotic spermatocytes and spermatids.
The presence of Fas (CD95) on ejaculated human sperm and its relation to apoptosis were investigated.

Purpose of the Study:

To determine if Fas (CD95) is present on ejaculated human sperm.
To investigate the relationship between Fas on sperm and the apoptotic marker Syto16.
To assess the role of Fas in sperm apoptosis.

Main Methods:

Semen samples from 77 infertile men were analyzed using flow cytometry before and after preparation.
Multiparameter flow cytometry assessed sperm concentration, viability, apoptosis, leukocytes (CD45), and Fas (CD95).
Indirect immunofluorescence confirmed flow cytometry findings.

Main Results:

Mean CD95 positive cells were <1% with no difference between normozoospermic and non-normozoospermic men.
No correlation was found between apoptotic sperm and CD95 positive cell concentration.
Leukocytes, not sperm, expressed significant CD95, which was upregulated by activating anti-Fas antibody and betulinic acid.

Conclusions:

No detectable quantity of Fas (CD95) was found on human ejaculated sperm.
Fas expression is primarily on leukocytes in semen, not on sperm.
Betulinic acid induced apoptosis in sperm, but not via Fas.