Related Experiment Video
Updated: Jul 6, 2026
Quantification of Tumor Cell Adhesion in Lymph Node Cryosections
Published on: February 9, 2020
Serum level of intercellular adhesion molecule-1 in children with malignant lymphoma
Nabil Abdelrazik1, Manal Fouda, Mohammad Hosam El-Deen Zaghloul
1Department of Pediatrics, Pediatric Hematology, and Oncology, and Bone Marrow Transplantation Unit, Mansoura Faculty of Medicine, Mansoura University, Mansoura, Egypt. nabeelabdelrazik2003@yahoo.com
Insights
Serum soluble intercellular adhesion molecule-1 (s-ICAM-1) levels are elevated in children with lymphoma, correlating with disease aggressiveness and poorer outcomes. This marker may aid in managing pediatric lymphoma cases.
Area of Science:
- Pediatric Oncology
- Immunology
- Biomarker Discovery
Background:
- Soluble intercellular adhesion molecule-1 (s-ICAM-1) is implicated in immune responses and cancer progression.
- Elevated s-ICAM-1 levels have been observed in various malignancies.
Purpose of the Study:
- To measure serum s-ICAM-1 levels in children with newly diagnosed lymphoma.
- To correlate s-ICAM-1 levels with clinical stage, pathology, laboratory data, and patient outcomes in pediatric lymphoma.
Main Methods:
- Studied 35 children with lymphoma (Hodgkin's disease and Non-Hodgkin's lymphoma) and 8 healthy controls.
- Assessed complete blood count, liver function tests, lactate dehydrogenase (LDH), and serum s-ICAM-1 via ELISA.
- Performed lymph node biopsy, bone marrow examination, and patient follow-up for over 12 months.
Main Results:
- Serum s-ICAM-1 levels were significantly higher in lymphoma patients compared to controls (p < 0.000).
- Elevated s-ICAM-1 correlated with advanced stages and high-grade Non-Hodgkin's lymphoma (p < 0.008, 0.04).
- Positive correlations found between high s-ICAM-1 and increased LDH (Hodgkin's disease), ALT (NHL), B symptoms, and worse patient outcomes.
Conclusions:
- High serum s-ICAM-1 levels in pediatric lymphoma indicate tumor aggressiveness.
- Quantification of s-ICAM-1 may identify children with a worse prognosis.
- Serum s-ICAM-1 can serve as an additional disease-associated marker for clinical management in pediatric lymphoma.
Objective:
To estimate the serum levels of soluble intercellular adhesion molecule-1 (s-ICAM-1) in children newly diagnosed with lymphoma and to correlate levels of s-ICAM-1 in lymphoma patients with clinical stage, pathological types, clinical and laboratory data and patient outcome.
Subjects And Methods:
Thirty-five children with newly-diagnosed malignant lymphoma (Non-Hodgkin's lymphoma, NHL: 23), Hodgkin's disease (HD: 12), and 8 apparently healthy subjects of matched age and sex taken as a control group were studied. For the patients and control group, the following tests were performed: complete blood count, and the following biochemical investigations: liver function tests, lactate dehydrogenase (LDH), and soluble ICAM-1 estimation using ELISA. In addition, for patients, pathological examination of lymph node biopsy for pathological grading, bone marrow aspiration and biopsy were done. Patients were observed for over 12 months or until death.
Results:
Serum ICAM-1 increased more in HD and NHL than in the control group (p < 0.000); also s-ICAM-1 increased in advanced stages and high-grade NHL (p < 0.008, 0.04, respectively). LDH levels were higher in patients compared to controls (p < 0.000). There was a positive correlation between high levels of s-ICAM-1 and increased levels of LDH in HD (r = 0.72, p < 0.008) and a positive correlation between high levels of s-ICAM-1 and increased ALT in NHL patients. A positive correlation between s-ICAM-1 levels and the presence of B symptoms in HD and NHL, and a positive correlation between elevated s-ICAM-1 levels and worse outcome in HD and NHL were detected.
Conclusions:
The data indicate that in children with malignant lymphoma, high serum levels of ICAM-1 correlated with tumor aggressiveness, and quantification of s-ICAM-1 levels may identify a subgroup of children with worse prognosis. Therefore, detection of s-ICAM-1 levels in children with malignant lymphoma might represent an additional disease-associated marker for use in the clinical management of the patients.
Related Concept Videos
01:31Immunoglobulin-like Cell Adhesion Molecules
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
01:20Cell Adhesion Molecules - Types and Functions
CAM Families
The Integrin family of proteins is primarily involved in a...