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Updated: Jul 6, 2026
A Porcine Corneal Endothelial Organ Culture Model Using Split Corneal Buttons
Published on: October 6, 2019
[Using of Cells Analyser software in the study of image of corneal specular microscope endothelial samples]
Cinthia Mendonça de Melo1, Procópio Miguel Dos Santos, Regina Cândido Ribeiro Dos Santos
1Universidade de Brasília, Brasília, DF, Brasil. cinmelo@hotmail.com
Purpose:
To describe differences between number of cells, evaluated endothelial area and relative error comparing endothelial samples from one endothelial image with endothelial samples obtained by guidance of a specific software, with number of cells and images as many as necessary.
Methods:
A transversal study was performed, comparing the endothelial samples with and without intervention of the software. The sample consisted of 157 eyes. They were divided into 2 groups. Group 1 consisted of data of the first image and group 2 consisted of data from as many images as necessary for a complete examination guided by the Cells Analyser software (95% confidence interval and 0.05 relative error). The evaluated data were number of counted cells, equivalent evaluated field (mm2) and relative error. Student's t test with 99% confidence interval (p<0.01) was used.
Results:
The compared data showed statistically significant differences between groups 1 and 2 in cell count (p=4x10-24), evaluated endothelial field (p=2x10-18) and relative error (p=1x10-21).
Conclusion:
The study of the sample data with and [corrected] without intervention of the Cells Analyzer software showed them to be significantly different with greater samples and smaller sample errors, evidencing the importance of their use in order to obtain specular microscopic cornea tests with small errors (less than 5%), giving them representativeness and reproducibility [corrected]
