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Related Experiment Video

Updated: Jul 6, 2026

An Assay for Lateral Line Regeneration in Adult Zebrafish
09:38

An Assay for Lateral Line Regeneration in Adult Zebrafish

Published on: April 8, 2014

Using the zebrafish lateral line to screen for ototoxicity.

Lynn L Chiu1, Lisa L Cunningham, David W Raible

  • 1Department of Otolaryngology-Head and Neck Surgery, University of Washington, Box 356515, Seattle, WA 98195, USA.

Journal of the Association for Research in Otolaryngology : JARO
|April 15, 2008
PubMed
Summary

Zebrafish lateral line hair cells screened 1,040 FDA-approved drugs for ototoxicity. This identified 14 new compounds toxic to hair cells, validating zebrafish as a drug screening model.

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Area of Science:

  • Neuroscience
  • Toxicology
  • Developmental Biology

Background:

  • The zebrafish lateral line system offers accessible hair cells for toxicity studies, mirroring mammalian inner ear structures.
  • Current drug development lacks a standard ototoxicity screen, leaving many FDA-approved drugs' risks unknown.

Purpose of the Study:

  • To screen a library of 1,040 FDA-approved drugs for ototoxic effects using zebrafish hair cells.
  • To identify novel compounds with hair cell toxicity and validate the zebrafish model for ototoxicity screening.

Main Methods:

  • Zebrafish larvae hair cell nuclei were labeled with vital dye.
  • Larvae were exposed to drugs at 100 µM for 1 hour in 96-well plates.
  • Hair cell viability was assessed via fluorescence microscopy; dose-response curves were generated.

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Last Updated: Jul 6, 2026

An Assay for Lateral Line Regeneration in Adult Zebrafish
09:38

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Published on: April 8, 2014

Evaluating Toxicity of Chemicals using a Zebrafish Vibration Startle Response Screening System
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Evaluating Toxicity of Chemicals using a Zebrafish Vibration Startle Response Screening System

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02:52

Small Molecule Screening and Toxicity Testing in Early-stage Zebrafish Larvae

Published on: March 7, 2025

Main Results:

  • The screen successfully identified 7 known ototoxic drugs (e.g., cisplatin) and 14 novel ototoxic compounds.
  • Dose-response analyses confirmed hair cell toxicity for all 21 compounds.
  • Two novel compounds (pentamidine isethionate, propantheline bromide) induced dose-dependent hair cell loss in mouse utricle preparations.

Conclusions:

  • The zebrafish lateral line serves as a validated, high-throughput screening tool for identifying ototoxic drugs.
  • This method can uncover previously unknown ototoxic potentials of FDA-approved drugs.
  • Further investigation into novel ototoxic compounds identified in this screen is warranted.