Related Experiment Video
Updated: Oct 5, 2026

Imaging Spatial Reorganization of a MAPK Signaling Pathway Using the Tobacco Transient Expression System
Published on: March 20, 2016
The Arabidopsis functional homolog of the p34cdc2 protein kinase
P C Ferreira1, A S Hemerly, R Villarroel
1Laboratorium voor Genetica, Rijksuniversiteit Gent, Belgium.
Abstract:
The p34cdc2 protein kinase is a key component of the eukaryotic cell cycle, which is required for G1 to S-phase transition and for entry into mitosis. Using a 380-base pair DNA fragment obtained by polymerase chain reaction amplification from an Arabidopsis thaliana flower cDNA library as a probe, we isolated and sequenced a cdc2-homologous cDNA from Arabidopsis. The encoded polypeptide has extensive homology with cdc2-like kinases. Furthermore, when expressed in a CDC28ts Saccharomyces strain, it partially restores the capacity to grow at 36 degrees C, indicating that the plant cDNA is a functional homolog of the p34cdc2 kinase. Genomic hybridization demonstrated that there is one copy of the cdc2 gene per Arabidopsis haploid genome. Using RNA gel blot analysis, we found that cdc2 mRNA is present in all plant organs.
More Related Videos
Related Concept Videos
DNA Damage can Stall the Cell Cycle
Positive Regulator Molecules
Inhibition of Cdk Activity
Anaphase Promoting Complex
Cell Signaling in Plants
DNA Damage Can Stall the Cell Cycle

