Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

What is Metabolism?00:52

What is Metabolism?

132.2K
Overview
132.2K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

16.6K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
16.6K
Carbohydrate Metabolism01:36

Carbohydrate Metabolism

14.4K
Carbohydrates are polymers composed of molecules containing atoms of carbon, hydrogen and oxygen. One gram of carbohydrate can provide four kilo-calories of energy, which makes it the most efficient instant energy source.
Starch accounts for approximately 60% of the carbohydrates consumed by humans. Since amylase enzymes cannot function in the stomach's acidic environment, starch can only be digested in the mouth and small intestine. Simple sugars are found naturally in milk and fruits in...
14.4K
Gene Flow02:39

Gene Flow

38.0K
Gene flow is the transfer of genes among populations, resulting from either the dispersal of gametes or from the migration of individuals.
38.0K
Overview of Metabolism01:40

Overview of Metabolism

38.6K
Living cells constantly carry out various chemical reactions which are necessary for their proper functioning. These reactions are interlinked to one another via multiple pathways. The collection of these chemical reactions is known as metabolism.
Plant Metabolism
Sunlight, the primary source of energy in plants, is first absorbed by the chlorophyll pigments present in their leaves. Plants then use this energy to carry out photosynthesis, where water is oxidized into oxygen and carbon dioxide...
38.6K
Regulation of Metabolism01:19

Regulation of Metabolism

11.7K
Cellular needs and conditions vary from cell to cell and change within individual cells over time. For example, the required enzymes and energetic demands of stomach cells are different from those of fat storage cells, skin cells, blood cells, and nerve cells. Furthermore, a digestive cell works much harder to process and break down nutrients during the time that closely follows a meal compared with many hours after a meal. As these cellular demands and conditions vary, so do the amounts and...
11.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Latent Vasculopathy of a Peripheral Pulmonary Artery in FBN1-Related Marfan Syndrome.

Pathology international·2026
Same author

Circulating tumor-associated autoantibody signatures for diagnosis and prognosis in small-cell lung cancer and lung adenocarcinoma.

British journal of cancer·2026
Same author

The impact of FBN1 variant types on pregnancy-related aortic dissection in women with Marfan syndrome.

International journal of cardiology·2026
Same author

Double Mutations in the FLNA and MYH11 Genes Causing Familial Thoracic Aortic Aneurysm and Dissection: A Report of Two Cases.

Internal medicine (Tokyo, Japan)·2026
Same author

Genetic regulation across germline and somatic variation on the Y chromosome contributes to type 2 diabetes.

Nature medicine·2026
Same author

Integrating ECG Into the Mayo Score Enhances Genotype Prediction in Hypertrophic Cardiomyopathy.

Journal of the American Heart Association·2026

Related Experiment Video

Updated: Feb 10, 2026

High-Throughput Metabolic Profiling for Model Refinements of Microalgae
11:07

High-Throughput Metabolic Profiling for Model Refinements of Microalgae

Published on: December 4, 2021

4.3K

[AMPD genes and urate metabolism].

Hiroko Morisaki1, Takayuki Morisaki

  • 1Department of Bioscience, National Cardiovascular Center Research Institute.

Nihon Rinsho. Japanese Journal of Clinical Medicine
|April 16, 2008
PubMed
Summary

AMP deaminase (AMPD) regulates purine metabolism and cellular energy through AMP levels. AMPD deficiency impacts metabolic myopathy and adenosine signaling, potentially affecting systemic energy status via AMPK.

Area of Science:

  • Biochemistry
  • Metabolic pathways
  • Enzymology

Background:

  • AMP deaminase (AMPD) is crucial for purine and urate metabolism, converting AMP to IMP.
  • Three human AMPD isozymes (M, L, E/H) are encoded by distinct genes.
  • Deficiencies in AMPD1 (skeletal muscle) and AMPD3 (red blood cells) are known human genetic disorders.

Purpose of the Study:

  • To elucidate the role of AMPD in purine nucleotide regulation.
  • To investigate the link between AMPD activity, adenosine levels, and systemic metabolic control.
  • To understand the impact of AMPD on AMP-activated protein kinase (AMPK) signaling.

Main Methods:

  • Enzyme activity assays for AMPD.
  • Analysis of purine nucleotide pools (ATP, ADP, AMP, IMP).

More Related Videos

Measurement of Metabolic Rate in Drosophila using Respirometry
04:31

Measurement of Metabolic Rate in Drosophila using Respirometry

Published on: June 24, 2014

20.3K
Metabolic Profile Analysis of Zebrafish Embryos
05:41

Metabolic Profile Analysis of Zebrafish Embryos

Published on: January 14, 2013

20.6K

Related Experiment Videos

Last Updated: Feb 10, 2026

High-Throughput Metabolic Profiling for Model Refinements of Microalgae
11:07

High-Throughput Metabolic Profiling for Model Refinements of Microalgae

Published on: December 4, 2021

4.3K
Measurement of Metabolic Rate in Drosophila using Respirometry
04:31

Measurement of Metabolic Rate in Drosophila using Respirometry

Published on: June 24, 2014

20.3K
Metabolic Profile Analysis of Zebrafish Embryos
05:41

Metabolic Profile Analysis of Zebrafish Embryos

Published on: January 14, 2013

20.6K
  • Measurement of adenosine concentrations.
  • Assessment of AMPK activation status.

    • Main Results:

    • AMPD deficiency alters intracellular purine nucleotide concentrations.
    • Changes in AMPD activity affect adenosine bioavailability.
    • AMPD influences systemic energy sensing through modulation of AMPK activity.

    Conclusions:

    • AMPD plays a significant role in maintaining purine homeostasis.
    • Dysregulation of AMPD can impact cellular energy status and metabolic signaling.
    • Targeting AMPD may offer therapeutic potential for metabolic disorders.