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Updated: Jul 6, 2026
OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy
Published on: February 5, 2020
Semisynthesis of dimeric proteins by expressed protein ligation
Barbara Ziaco1, Soccorsa Pensato, Luca D D'Andrea
1Università di Napoli Federico II, Dipartimento delle Scienze Biologiche, Facoltà di Scienze Biotecnologiche, via Mezzocannone 16, 80134 Napoli, Italy.
Abstract:
A one-pot synthesis of homodimeric proteins is described. The synthetic strategy is based on a double expressed protein ligation reaction between thioester peptides and a new bis-cysteinyl linker. The protocol was also applied to the synthesis of heterodimers.
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