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Studies on leukotriene D4 as an eosinophil chemoattractant
D F Woodward1, A H Krauss, A L Nieves
1Department of Biological Sciences, Allergan Inc., Irvine, CA 92713-9534.
Drugs Under Experimental and Clinical Research
|January 1, 1991
Summary
Leukotriene D4 (LTD4) acts as a potent chemoattractant, driving eosinophil infiltration in the conjunctiva. This effect is tissue-dependent, highlighting LTD4's role in allergic responses.
Area of Science:
- Immunology
- Allergy Research
- Ocular Inflammation
Background:
- Eosinophils play a key role in allergic inflammation.
- Leukotriene D4 (LTD4) is implicated in allergic responses.
- Understanding eosinophil chemoattractants is crucial for allergy treatment.
Purpose of the Study:
- To investigate the chemoattractant properties of LTD4 on eosinophils in vivo.
- To determine the role of LTD4 in eosinophil recruitment to ocular and cutaneous tissues.
- To evaluate the efficacy of LTD4 as an eosinophil chemoattractant compared to other mediators.
Main Methods:
- Utilized guinea pigs for in vivo studies.
- Quantified eosinophil infiltration using digital image analysis of stained tissue sections.
- Administered LTD4 topically to the ocular surface and intradermally to the skin.
- Investigated the effect of LT-antagonist SK&F 104353 on LTD4-induced infiltration.
Main Results:
- Topical LTD4 induced significant eosinophil infiltration into the conjunctival epithelium.
- LTD4 was more potent and efficacious than other tested mediators in the conjunctiva.
- LT-antagonist SK&F 104353 blocked LTD4-induced conjunctival eosinophil infiltration.
- LTD4 did not induce eosinophil infiltration in other ocular tissues or skin, despite increasing vascular permeability.
Conclusions:
- LTD4 acts as a potent eosinophil chemoattractant in the conjunctiva.
- Eosinophil emigration induced by LTD4 is regulated in a tissue-dependent manner.
- These findings suggest LTD4's specific role in ocular allergic inflammation.