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Related Experiment Video

Updated: Jul 5, 2026

Differentiation of Mouse Breast Epithelial HC11 and EpH4 Cells

Differentiation of Mouse Breast Epithelial HC11 and EpH4 Cells

Published on: February 27, 2020

HER2-dependent MMP-7 expression is mediated by activated STAT3.

Guogang Yuan¹, Lu Qian, Ming Shi

¹Institute of Basic Medical Sciences, Beijing 100850, PR China.

Cellular Signalling

|April 16, 2008

Summary

Overexpression of HER2 (Human Epidermal growth factor Receptor 2) increases Matrix Metalloproteinase-7 (MMP-7) expression via STAT3 activation. This HER2/STAT3 pathway is crucial for understanding early tumor development and MMP-7 regulation.

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Differentiation of Mouse Breast Epithelial HC11 and EpH4 Cells

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Exploring the Pharmacological Action and Molecular Mechanism of Salidroside in Inhibiting MCF-7 Cell Proliferation and Migration

Exploring the Pharmacological Action and Molecular Mechanism of Salidroside in Inhibiting MCF-7 Cell Proliferation and Migration

Published on: June 9, 2023

Area of Science:

Molecular Biology
Cancer Research
Gene Regulation

Background:

Matrix Metalloproteinase-7 (MMP-7) expression is tightly controlled transcriptionally.
Understanding MMP-7 gene regulation is vital for elucidating its overexpression in early tumor development.

Purpose of the Study:

To investigate the regulatory mechanisms of MMP-7 expression, particularly in the context of HER2 overexpression.
To determine the role of STAT3 signaling in HER2-mediated MMP-7 upregulation.

Main Methods:

Utilized MCF-7 cells stably overexpressing HER2 (MCF-7/HER2) and constitutively active STAT3 (MCF-7/STAT3C).
Assessed MMP-7 mRNA and protein levels using RT-PCR and Western blot.
Analyzed MMP-7 promoter activity via reporter assays.
Verified STAT3 binding to the MMP-7 promoter using Chromatin Immunoprecipitation (ChIP) and site-directed mutagenesis.

Main Results:

MMP-7 mRNA and protein expression were increased in MCF-7/HER2 and MCF-7/STAT3C cells.
MMP-7 promoter activity was upregulated in MCF-7/HER2 cells and further enhanced by HRG induction.
Activated STAT3 directly bound to the MMP-7 promoter, driving its transcriptional upregulation.
STAT3 phosphorylation was elevated in HER2-overexpressing and HRG-induced cells.

Conclusions:

HER2-dependent transcriptional upregulation of MMP-7 is mediated by activated STAT3.
The HER2/STAT3 signaling pathway plays a critical role in controlling MMP-7 expression.
This pathway is a potential target for therapeutic interventions in cancers with MMP-7 overexpression.