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In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity
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Engineering of protease variants exhibiting altered substrate specificity.

Saravanan Sellamuthu1, Bae Hyun Shin, Eui Seung Lee

  • 1Department of Life Science, Gwangju Institute of Science and Technology, 1 Oryong-dong, Buk-gu, Gwangju 500-712, Republic of Korea.

Biochemical and Biophysical Research Communications
|April 17, 2008
PubMed
Summary

Researchers engineered a new genetic screening system to alter the P2 specificity of Hepatitis A virus 3C protease (HAV 3CP). This method successfully identified variants with improved Gln-over-Thr cleavage at the P2 position, demonstrating effective protease engineering.

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