Related Experiment Video
Updated: Jul 5, 2026

Applying an Inducible Expression System to Study Interference of Bacterial Virulence Factors with Intracellular Signaling
Published on: June 25, 2015
In vitro signaling by MAPK and NFkappaB pathways inhibited by Yersinia YopJ
1University of Texas Southwestern Medical Center, Dallas, Texas, USA.
Abstract:
A procedure for an in vitro signaling assay is described for the MAPK and NFkappaB pathways. The method uses a membrane-cleared lysate that contains all the soluble components required for activating these signaling cascades. The pathways can be activated by variety of molecules, including kinases, G-proteins, and E3 ligases. We demonstrate that YopJ inhibits downstream of all these activators. The in vitro signaling assay is ideal for initial biochemical studies on activators and inhibitors of the MAPK and NFkappaB pathways.
Related Concept Videos
Interactions Between Signaling Pathways
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
MAPK Signaling Cascades
cAMP-dependent Protein Kinase Pathways
The JAK-STAT Signaling Pathway
Inhibitors of Viral Protein Synthesis
Inhibition of Cdk Activity

