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MISSION esiRNA for RNAi Screening in Mammalian Cells
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MISSION esiRNA for RNAi Screening in Mammalian Cells

Published on: May 12, 2010

A method with flexible and balanced control of false negatives and false positives for hit selection in RNA

Edna Schechtman1

  • 1Department of Industrial Engineering and Management, Ben Gurion University, Beer Sheva, Israel. ednas@bgumail.bgu.ac.il

Journal of Biomolecular Screening
|April 17, 2008
PubMed

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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...