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Updated: Jul 5, 2026

Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor
Published on: October 15, 2015
Use of poly-beta-hydroxy-butyrate as a slow-release electron donor for the microbial reductive dechlorination of TCE
1Department of Chemistry "Stanislao Cannizzaro", University of Rome La Sapienza, Piazzale Aldo Moro 5, 00185 Rome, Italy.
Abstract:
In situ anaerobic reductive dechlorination, using slow-release electron donors, is emerging as an effective and sustainable (low-cost and low-maintenance) technology to remediate aquifers contaminated by chloroethenes. In the present study, we investigated the use of poly-beta-hydroxy-butyrate (PHB), a fully biodegradable polymer, as a slow-release source of hydrogen and acetate for the reductive dechlorination of trichloroethene (TCE). Results of this study indicated that TCE dechlorination in PHB-amended microcosms was 2.3-times higher than in non-amended controls. This higher activity was explained by a higher H(2) level in PHB-amended microcosms. As usual, acetate was the major sink (approximately 90%) of reducing equivalents available from PHB degradation, whereas no acetotrophic dechlorination was observed.
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