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Two competitive enzyme immunoassays for the detection of IgG class antibodies to hepatitis A antigen
C L Vitral1, A M Gaspar, C F Yoshida
1Departamento of Virology, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil.
Revista Da Sociedade Brasileira De Medicina Tropical
|April 1, 1991
Abstract:
Two competitive enzyme immunoassays (EIA) techniques were developed: in the first (COMP-1), test sera were added together with HAV antigen on anti-HAV IgG-coated wells followed by an anti-HAV HRP conjugate; in the second (COMP-2), test sera and anti-HAV HRP conjugate competed for HAV epitopes previously adsorbed to anti-HAV IgG-coated wells. Both procedures used tetramethylbenzidine (TMB) as a substrate. Both competitive tests were shown to be reproducible and suitable for routine diagnosis and research purposes.