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Related Experiment Videos

Electrospray ionization for analysis of platelet-activating factor.

S T Weintraub1, R N Pinckard, M Hail

  • 1Department of Pathology, University of Texas Health Science Center, San Antonio 78284-7750.

Rapid Communications in Mass Spectrometry : RCM
|July 1, 1991
PubMed
Summary

Electrospray-ionization mass spectrometry effectively analyzed Platelet-activating factor (PAF). Increased voltage fragmented PAF, revealing key molecular components and aiding structural interpretation.

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Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Mass Spectrometry

Background:

  • Platelet-activating factor (PAF) is a critical bioactive phospholipid.
  • Accurate structural analysis of PAF is essential for understanding its biological roles.
  • Electrospray-ionization mass spectrometry (ESI-MS) is a powerful tool for analyzing lipids.

Purpose of the Study:

  • To analyze Platelet-activating factor (PAF) using electrospray-ionization mass spectrometry (ESI-MS).
  • To investigate the fragmentation patterns of PAF under varying ESI conditions.
  • To confirm structural interpretations using tandem mass spectrometry.

Main Methods:

  • Analysis of PAF using a single quadrupole mass spectrometer with electrospray ionization.
  • Optimization of capillary exit voltage to induce and control fragmentation.

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  • Positive- and negative-ion detection modes were employed.
  • Verification of fragmentation pathways using ESI tandem mass spectrometry on a triple-quadrupole instrument.

    • Main Results:

    • Positive-ion mode: sodiated molecule ([M+Na]+) was dominant at low voltage; increased voltage induced fragmentation, notably loss of trimethylamine ([M+Na-59]+).
    • Key fragment ions at m/z 147 (loss of trimethylamine from phosphocholine) and m/z 184 (protonated phosphocholine) were observed.
    • Negative-ion mode: the primary species detected was the chloride adduct ([M+Cl]-).

    Conclusions:

    • ESI-MS, particularly with controlled voltage, is suitable for analyzing PAF.
    • Fragmentation patterns provide valuable information for elucidating PAF structure.
    • Tandem mass spectrometry confirms the proposed fragmentation pathways and structural assignments.