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Isolation and Screening from Soil Biodiversity for Fungi Involved in the Degradation of Recalcitrant Materials
Published on: May 16, 2022
Molecular characterization of multiple xylanase producing thermophilic/thermotolerant fungi isolated from composting
1Department of Microbiology, Guru Nanak Dev University, Amritsar, Punjab, India.
Aims:
Molecular characterization of commercially important group of xylanase producing thermophilic/thermotolerant fungi.
Methods And Results:
DNA from 16 thermophilic/thermotolerant fungal isolates was amplified by PCR using three sets of primers: (i) internal transcribed spacer sequence (ITSI-5.8S-ITSII), (ii) D1/D2 hyper variable region of 26S rDNA and (iii) 18S rDNA region. The amplified products of internal transcribed spacers (ITS) and D1/D2 region were sequenced and analysed using CLUSTALX, whereas, amplified 18S rDNA region was subjected to RFLP analysis based on restriction digestion with RsaI, MboI and Hinf I.
Conclusions:
The sequence based analyses of ITSI-5.8S-ITSII as compared with D1/D2 region of 26-28S rDNA was found to be a better tool for phylogenetic resolution of thermophilic/thermotolerant fungi. The ITSI-5.8S-ITSII sequence-based dendrogram indicates an early divergence of the alkaline active xylanase producing thermophilic fungal strains.
Significance And Impact Of The Study:
This study was the first report on phylogenetic characterization of thermophilic/thermotolerant fungi.
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