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Photo-CIDNP study of the interaction between the glucocorticoid receptor DNA-binding domain and glucocorticoid
E Kellenbach1, T Härd, R Boelens
1Department of Chemistry, University of Utrecht, The Netherlands.
Journal of Biomolecular NMR
|May 1, 1991
Abstract:
Photo-CIDNP studies were performed on two protein fragments that both contain the double zinc-finger DNA-binding domain of the glucocorticoid receptor. In the absence of DNA, Tyr452 and Tyr474 are polarised in both fragments while Tyr497 is not. Addition of a palindromic glucocorticoid response element (GRE) results in the suppression of Tyr474 polarization while the polarization of Tyr452 is unaffected. The same result is observed upon adding a half GRE to the protein fragment indicating that the suppression of Tyr474 polarization is not due to protein-protein contacts but to interaction with DNA.