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Related Experiment Videos

Plant enolase: gene structure, expression, and evolution.

D Van der Straeten1, R A Rodrigues-Pousada, H M Goodman

  • 1Laboratorium voor Genetica, Rijksuniversiteit Gent, Belgium.

The Plant Cell
|July 1, 1991
PubMed
Summary
This summary is machine-generated.

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Plant enolase genes show conservation with a unique insertion. Expression varies by tissue and ripening, but not by anaerobiosis or heat shock, suggesting alternative glycolytic pathways in chloroplasts.

Area of Science:

  • Plant molecular biology
  • Enzyme kinetics
  • Biochemistry

Background:

  • Enolase is a key enzyme in glycolysis.
  • Understanding plant enolase gene expression and function is crucial for metabolic studies.
  • Previous research has focused on enolase in non-plant systems.

Purpose of the Study:

  • To clone and characterize enolase genes from tomato and Arabidopsis.
  • To investigate the expression patterns of enolase genes under various physiological conditions.
  • To explore the role of enolase in plant metabolism, particularly in chloroplasts.

Main Methods:

  • Gene cloning and sequence analysis of plant enolases.
  • Messenger RNA (mRNA) quantification using molecular techniques.
  • Enzyme activity assays.

Related Experiment Videos

  • Comparative analysis with enolases from other organisms.

    • Main Results:

    • Plant enolases exhibit high conservation with a unique 5-amino acid insertion.
    • Enolase expression and activity are higher in roots than in green tissues under normal conditions.
    • Artificial ripening in tomato fruits significantly induces enolase mRNA.
    • Anaerobiosis and heat shock have minimal impact on enolase mRNA levels.
    • Evidence suggests a substrate shuttle mechanism in Arabidopsis chloroplasts, challenging the presence of a complete glycolytic enzyme set.

    Conclusions:

    • Plant enolases possess unique structural features.
    • Enolase expression is differentially regulated in plants based on tissue type and developmental stage.
    • The glycolytic pathway in Arabidopsis chloroplasts may involve a substrate shuttle rather than a complete enzyme set.