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Updated: Jul 5, 2026

Absolute Quantification of Cell-Free Protein Synthesis Metabolism by Reversed-Phase Liquid Chromatography-Mass Spectrometry
Published on: October 25, 2019
Quantitative analysis of natural cyclodextrins by high-performance liquid chromatography with pulsed amperometric
Tarja Toropainen1, Pekka Jarho, Marko Lehtonen
1Department of Pharmaceutical Chemistry, University of Kuopio, Finland. tarja.toropainen@uku.fi
Abstract:
Simple HPLC-PAD methods were developed for quantitation of cyclodextrins (CDs) in aqueous matrices from in vitro cell permeation studies. C-18 solid-phase extraction was used for sample pretreatment. Samples were analysed using acetonitrile-water mobile phase with post-column alkalization by 0.5M NaOH. Zorbax SB-Aq (for alpha-CD) and Zorbax SB-Phenyl (for beta-CD and gamma-CD) columns gave excellent peak shape and sufficient resolution of CD to glucose (2.7-3.2). The methods showed good concentration-response relationship (r > or = 0.999), precision (RSD% 0.7-5.1), repeatability (RSD% 3.4-13.7) and accuracy (87-107%). The limits of quantitation were 0.78, 0.46 and 0.52 microg/ml for alpha-CD, beta-CD and gamma-CD (RSD% of 10.6, 8.1 and 16.3, respectively).
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