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TNFalpha -308 C-->t and -863 C-->a polymorphisms and spermiogram characteristics
Christine Kurz1, Eva-Katrin Bentz, Dominik Denschlag
1Department of Gynecologic Endocrinology and Reproductive Medicine, University of Vienna School of Medicine, Vienna, Austria.
Gynecologic and Obstetric Investigation
|April 18, 2008
Summary
Genetic variations in TNFalpha, specifically the -308 C-->T and -863 C-->A polymorphisms, were investigated in relation to male infertility. These TNFalpha polymorphisms were found to be not associated with semen quality or male infertility in Caucasian men.
Area of Science:
- Genetics
- Reproductive Medicine
- Molecular Biology
Background:
- Genetic factors are implicated in the etiology of male infertility.
- Tumor Necrosis Factor alpha (TNFalpha) gene polymorphisms are potential contributors to reproductive health outcomes.
Purpose of the Study:
- To investigate the association between TNFalpha gene polymorphisms (-308 C-->T and -863 C-->A) and male infertility.
- To determine if these polymorphisms correlate with semen parameters.
Main Methods:
- A prospective case-control study involving 577 Caucasian men from an infertility clinic.
- Analysis of TNFalpha -308 C-->T and -863 C-->A polymorphisms using PCR on sperm DNA.
- Correlation of allele and genotype frequencies with spermiogram results.
Main Results:
- No significant differences in allele or genotype frequencies of TNFalpha -308 C-->T and -863 C-->A polymorphisms were observed between normozoospermic and non-normozoospermic men.
- Mutant alleles were not found to be overrepresented in subgroups with specific infertility syndromes like oligoasthenoteratozoospermia.
Conclusions:
- The studied TNFalpha polymorphisms (-308 C-->T and -863 C-->A) are not associated with variations in spermiogram characteristics.
- These TNFalpha polymorphisms do not serve as molecular markers for genetic susceptibility to male infertility.
