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Related Concept Videos

Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
Replication in Eukaryotes02:31

Replication in Eukaryotes

Overview
Replication in Eukaryotes02:31

Replication in Eukaryotes

Overview
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
Chromosome Replication02:31

Chromosome Replication

Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins.  This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin of...
Replication in Prokaryotes01:32

Replication in Prokaryotes

DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...

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Related Experiment Video

Updated: Jul 5, 2026

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
06:40

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

Controlled rereplication at DNA replication origins.

María Gómez1

  • 1Instituto de Microbiología Bioquímica, CSIC/Universidad de Salamanca, Edificio Departamental, Salamanca, Spain. mgvf@usal.es

Cell Cycle (Georgetown, Tex.)
|April 18, 2008
PubMed
Summary

Researchers discovered repeated synthesis of small DNA fragments at human replication origins during S phase. This finding offers a new perspective on regulating DNA replication initiation and maintaining genome stability.

Related Experiment Videos

Last Updated: Jul 5, 2026

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
06:40

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Cellular DNA replication must initiate only once per cell cycle to ensure genome stability.
  • Replication origins are critical sites for initiating DNA synthesis during S phase.
  • Dysregulation of DNA replication can lead to genomic instability and cell death.

Purpose of the Study:

  • To investigate the events occurring at human DNA replication origins during origin firing.
  • To identify any novel DNA synthesis activities associated with replication initiation.
  • To explore the implications of these findings for understanding replication origin regulation.

Main Methods:

  • Observation of DNA synthesis at human replication origins during S phase in normal cells.
  • Characterization of the size and location of newly synthesized DNA fragments.
  • Correlation of DNA fragment synthesis with replication origin activity (firing).

Main Results:

  • Discovery of repeated synthesis of small genome fragments (approx. 200 bp) at human DNA replication origins.
  • These fragments are synthesized concurrently with origin firing during S phase.
  • The generation of these rereplicated DNA fragments is closely linked to replication origin activity.

Conclusions:

  • The study reveals an unexpected DNA synthesis event intimately associated with replication origin initiation.
  • This finding challenges the traditional view of replication origins and suggests a novel mechanism.
  • The discovery provides a new framework for studying the regulation of DNA replication origins and genome stability.