Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Thrombin regulates CD40 expression in microglial cells.

Jonathan R Weinstein1, Russell E Ettinger, Matthew Zhang

  • 1Department of Neurology, School of Medicine, University of Washington, Seattle, Washington 98195, USA. jweinste@u.washington.edu

Neuroreport
|April 18, 2008
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Patient-Reported Outcomes and Body Mass Index in Adolescents and Young Adults After Gender-Affirming Chest Surgery: A Single-Center Cross-Sectional Study.

Annals of surgery open : perspectives of surgical history, education, and clinical approaches·2026
Same author

Microchimerism in the human brain, quantitative assessment and single nuclei profiling establish cell types and diversity.

bioRxiv : the preprint server for biology·2026
Same author

The Nordic Experience of Pediatric Heart-Lung Transplantation.

Clinical transplantation·2026
Same author

Unilateral Cleft Lip: Primary Repair.

Oral and maxillofacial surgery clinics of North America·2026
Same author

Bulbospongiosus Muscle Flap for Repair of Rectal Injury During Penile Inversion Vaginoplasty.

Plastic and reconstructive surgery. Global open·2026
Same author

Current availability and status of paediatric cardiac transplantation and mechanical circulatory support in twenty-eight European countries.

European journal of pediatrics·2026
Same journal

Electroacupuncture alleviates neuroinflammation and promotes recovery of neurological functions after intracerebral hemorrhage by modulating α7nAChR/JAK2/STAT3 signaling pathway.

Neuroreport·2026
Same journal

Non-cell-autonomous regulation of Bhlhb5 expression in cortical projection neurons by GABAergic interneuron development and position.

Neuroreport·2026
Same journal

C-C motif chemokine ligand 3 mediates inflammatory response via NLRP3 inflammasome and neuron damage after traumatic brain injury.

Neuroreport·2026
Same journal

Methyltransferase-like 14 alleviates neuronal ferroptosis in Alzheimer's disease by regulating the peroxiredoxin 6/apoptosis signal-regulating kinase 1 signaling pathway.

Neuroreport·2026
Same journal

Hand mental rotation reaction time reflects motor imagery strategy and predicts changes in finger dexterity after motor imagery.

Neuroreport·2026
Same journal

Functional exploration of metabotropic glycine receptors in cultured rat hippocampal slices.

Neuroreport·2026
See all related articles

Thrombin activates microglial cells, increasing CD40 expression in a dose-dependent manner. This finding suggests a role for thrombin in central nervous system inflammatory diseases like stroke.

Area of Science:

  • Neuroimmunology
  • Cellular Biology
  • Biochemistry

Background:

  • Microglial cells are key innate immune cells in the central nervous system.
  • They rapidly respond to injury via proliferation, cytokine release, and antigen expression.
  • Thrombin, a serine proteinase, can activate microglial cells.

Purpose of the Study:

  • To investigate the effect of thrombin on microglial cell activation.
  • To determine the role of thrombin in regulating CD40 expression on microglial cells.

Main Methods:

  • Utilized N9 microglial cells for experiments.
  • Administered pharmaceutical-grade thrombin and measured CD40 expression.
  • Employed thrombin inhibitors, thrombin receptor-activating peptide, and MAPK pathway inhibitors.

Related Experiment Videos

Main Results:

  • Thrombin dose-dependently increased CD40 expression in N9 microglial cells.
  • Thrombin inhibition blocked this effect.
  • Thrombin receptor-activating peptide mimicked the effect.
  • Mitogen-activated protein kinase pathway inhibitors modified the response.

Conclusions:

  • Thrombin significantly upregulates CD40 expression on microglial cells.
  • This interaction is mediated through thrombin receptors and MAPK pathways.
  • Thrombin-induced CD40 regulation may contribute to neuroinflammation in conditions like multiple sclerosis and stroke.