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Mutant strains of Streptomyces cinnamonensis protoplasts. Cultural and physiological conditions
J Matĕjů1, J Marsálková, M Nohýnek
1Institute of Microbiology, Czechoslovak Academy of Sciences, Prague.
Folia Microbiologica
|January 1, 1991
Abstract:
Mycelium of Streptomyces cinnamonensis mutant strains cultivated in a synthetic medium with glycine produced protoplasts after lysis of cell walls with lysozyme. The protoplast yield was up to 95%. The protoplasts could revert and mycelial forms were thus regenerated. In a sucrose-containing medium the protoplasts stored at 4 degrees C were stable for 2 d.