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Updated: Jul 5, 2026
Rodent Model of Intestinal Ischemia-Reperfusion Injury via Occlusion of the Superior Mesenteric Artery
Published on: October 20, 2023
Isoflurane attenuates myoglobin release during ischemic and/or reperfusion periods
H Kitagawa1, T Yamazaki, T Akiyama
1Department of Anesthesiology, Shiga University of Medical Science, Shiga, Japan. hirotosi@belle.shiga-med.ac.jp
Background:
Recently, we have developed cardiac microdialysis for detection of protein leakage from the injured myocardium. We examined whether the exposures to isoflurane would exert a beneficial effect on myocardial injury caused by ischemia or reperfusion.
Methods:
A dialysis probe was implanted into the left ventricle free wall in the rabbits. The dialysate myoglobin level served as an index of myocardial interstitial myoglobin levels. Rabbits were randomly assigned to one of three groups: (1) without exposure to isoflurane (vehicle, n=6), (2) inhale 1 MAC isoflurane once for 30 min (ISO30-1, n=6), and (3) twice for 30 min (ISO30-2, n=6). All rabbits underwent 30 min of coronary occlusion and 60 min of reperfusion. To determine whether the isoflurane induced myocardial protection against chemical hypoxia, sodium cyanide (30 mM) was administered and dialysate myoglobin levels were measured with (n=6) and without pre-exposure to isoflurane twice for 30 min (n=6).
Results:
In all three groups dialysate myoglobin levels were increased by coronary occlusion and furthermore augmented by reperfusion. In comparison with the vehicle group, the ISO30-1 group suppressed only the increase in the dialysate myoglobin level during reperfusion. The ISO30-2 group suppressed during both the ischemic and reperfusion periods. Cyanide induced increases in dialysate myoglobin levels. These increments in dialysate myoglobin levels were suppressed by repeated exposure to isoflurane.
Conclusion:
Repeated exposure to isoflurane suppressed myocardial myoglobin release caused by both ischemia and reperfusion injury. Isoflurane may provide protection against myocardial ischemia/reperfusion and hypoxic injuries.
Insights
Repeated exposure to isoflurane reduced myocardial injury from ischemia and reperfusion. This anesthetic may protect against heart damage and hypoxic injuries.
Area of Science:
- Cardiology
- Anesthesiology
- Biomedical Engineering
Background:
- Cardiac microdialysis is a novel method for detecting protein leakage in injured heart muscle.
- Investigated the potential protective effects of isoflurane on myocardial injury induced by ischemia and reperfusion.
Purpose of the Study:
- To evaluate the efficacy of isoflurane in mitigating myocardial ischemia/reperfusion injury.
- To assess isoflurane's protective role against chemical hypoxia-induced myocardial injury.
Main Methods:
- Cardiac microdialysis was used in rabbits to measure interstitial myoglobin levels, an indicator of myocardial injury.
- Rabbits were exposed to isoflurane once or twice before undergoing coronary occlusion and reperfusion.
- Chemical hypoxia was induced using sodium cyanide to further assess isoflurane's protective effects.
Main Results:
- Myoglobin levels increased significantly during ischemia and reperfusion in all groups.
- Repeated isoflurane exposure (twice) suppressed myoglobin release during both ischemic and reperfusion phases.
- Isoflurane pre-exposure attenuated the myoglobin release caused by sodium cyanide-induced chemical hypoxia.
Conclusions:
- Repeated isoflurane administration offers significant protection against myocardial ischemia/reperfusion injury.
- Isoflurane demonstrates potential as a cardioprotective agent against both ischemic and hypoxic damage.
