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The C, V and W proteins of Nipah virus inhibit minigenome replication

Katrina Sleeman1, Bettina Bankamp1, Kimberly B Hummel1

  • 1Measles, Mumps, Rubella, and Herpesvirus Laboratory Branch, Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA.

The Journal of General Virology
|April 19, 2008
PubMed

Insights

Nipah virus (NiV) proteins C, V, and W inhibit gene expression and replication. These proteins from NiV, measles virus, and human parainfluenzavirus 3 show cross-species inhibitory functions, suggesting shared properties.

Area of Science:

  • Virology
  • Molecular Biology
  • Genetics

Background:

  • Nipah virus (NiV) is a highly pathogenic zoonotic virus.
  • The NiV phosphoprotein (P) gene encodes accessory proteins C, V, and W.
  • These proteins are implicated in viral replication and pathogenesis.

Purpose of the Study:

  • To investigate the functional properties of NiV C, V, and W proteins.
  • To determine their ability to inhibit gene expression and viral replication.
  • To explore potential functional similarities with related paramyxoviruses.

Main Methods:

  • Plasmid-based minigenome replication assays were used.
  • Chloramphenicol acetyltransferase (CAT) reporter gene expression was measured.
  • Inhibition assays were performed using NiV, measles virus (MV), and human parainfluenzavirus 3 (hPIV3) minigenomes.

Main Results:

  • NiV C, V, and W proteins dose-dependently inhibited CAT expression.
  • NiV C, V, and W proteins inhibited replication of NiV and MV minigenomes, but not hPIV3.
  • MV C and V proteins inhibited NiV minigenome replication, while hPIV3 C protein inhibited replication of all three viruses.

Conclusions:

  • NiV C, V, and W proteins possess inhibitory functions on viral replication.
  • Despite limited sequence similarity, these proteins exhibit cross-species inhibitory activity.
  • This suggests conserved functional properties among paramyxovirus accessory proteins.

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