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Related Experiment Video

Updated: Jul 5, 2026

Purification of HLA-G+ Extravillous Trophoblasts from Human Term Placental Tissues for Phenotyping and Functional Analysis
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Differential gene expression profiling in HELLP syndrome placentas.

Bum-Yong Kang1, Stephen Tsoi, Shan Zhu

  • 1Department of Obstetrics and Gynecology, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA.

Reproductive Sciences (Thousand Oaks, Calif.)
|April 19, 2008
PubMed
Summary
This summary is machine-generated.

This study identified downregulated genes in HELLP placentas using suppression subtractive hybridization. SERPINE2 protein levels were significantly decreased, suggesting its potential as a biomarker for early HELLP syndrome diagnosis.

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Last Updated: Jul 5, 2026

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Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • HELLP syndrome is a severe pregnancy complication.
  • Identifying molecular differences in HELLP placentas is crucial for understanding its pathophysiology.
  • Gene expression profiling can reveal key molecular changes.

Purpose of the Study:

  • To identify differentially expressed genes in placentas from patients with HELLP syndrome.
  • To investigate the role of specific genes and proteins in HELLP pathophysiology.
  • To explore potential biomarkers for early diagnosis of HELLP syndrome.

Main Methods:

  • Suppression subtractive hybridization (SSH) was used to compare gene expression between HELLP and non-HELLP placentas.
  • cDNA libraries were constructed and clones were sequenced.
  • Real-time quantitative polymerase chain reaction (qPCR) and Western blot analysis were employed for validation.

Main Results:

  • 37 differentially expressed genes were identified, involved in various cellular processes.
  • No significant differences were found for selected upregulated genes.
  • Six downregulated genes were significantly suppressed in HELLP placentas, including CTHRC1 and SERPINE2.
  • SERPINE2 protein levels were significantly decreased in HELLP placentas.

Conclusions:

  • SERPINE2 is significantly downregulated in HELLP placentas.
  • SERPINE2 (PAI-1) shows potential as a biomarker for the early diagnosis of HELLP syndrome.
  • Further clinical testing of SERPINE2 is warranted for HELLP syndrome diagnosis.