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Updated: Jul 5, 2026

Characterization of Biological Absorption Spectra Spanning the Visible to the Short-Wave Infrared
Published on: January 10, 2025
[Measurement and analysis of absorption spectrum of human blood]
Zhi-Min Zhao1, Yu-Jun Xin, Le-Xin Wang
1College of Science, Nanjing University of Aeronautics & Astronautics, Nanjing 210016, China. zhaozhimin@nuaa.edu.cn
Abstract:
The present paper puts forward a method of disease diagnosis by using the technology of spectrum analysis of human blood serum. The generation mechanism of absorption spectrum is explained and the absorption spectra of the normal blood serum and the sick blood serum are listed from the experiments of absorption spectrometry. Though the value of absorbency of the sick blood serum is almost equal to that of the normal blood serum in the most absorption spectra, there are some differences around 278 nm in the absorption spectrum. The absorbency of the blood serum with hyperglycemia is greater than that of the normal blood serum at 285 nm in the spectrum, and besides, there comes a peak shift of absorption with hyperglycemia. In the absorption spectrum of the blood serum with hypercholesterolemia, there is a clear absorption peak at 414 nm. However there is not any peak at that wavelength in the absorption spectrum of the normal blood serum. Through comparing the characters of the spectrum, we can judge if the blood sample is or not, and this blood analysis is a new method for the diagnosis of disease. Compared with other methods of blood measurements, the method of absorption spectrum analysis of blood serum presented in this paper, is more convenient for measurement, simpler for analysis, and easier to popularize.
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