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Related Experiment Video

Updated: Jul 5, 2026

Microfluidic Chips for In Situ Crystal X-ray Diffraction and In Situ Dynamic Light Scattering for Serial Crystallography
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Microfluidic Chips for In Situ Crystal X-ray Diffraction and In Situ Dynamic Light Scattering for Serial Crystallography

Published on: April 24, 2018

High-throughput small angle X-ray scattering from proteins in solution using a microfluidic front-end.

K Nørgaard Toft1, Bente Vestergaard, Søren S Nielsen

  • 1Departments of Medicinal Chemistry, University of Copenhagen, Denmark. knt@farma.ku.dk

Analytical Chemistry
|April 22, 2008
PubMed
Summary

Researchers developed an automated microfluidic chip for high-throughput Small-Angle X-ray Scattering (SAXS) analysis of biomolecules. This novel system enables rapid structural studies in solution, even with minimal sample volumes.

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Last Updated: Jul 5, 2026

Microfluidic Chips for In Situ Crystal X-ray Diffraction and In Situ Dynamic Light Scattering for Serial Crystallography
11:48

Microfluidic Chips for In Situ Crystal X-ray Diffraction and In Situ Dynamic Light Scattering for Serial Crystallography

Published on: April 24, 2018

Crystallization of Proteins on Chip by Microdialysis for In Situ X-ray Diffraction Studies
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Crystallization of Proteins on Chip by Microdialysis for In Situ X-ray Diffraction Studies

Published on: April 11, 2021

Small and Wide Angle X-Ray Scattering Studies of Biological Macromolecules in Solution
12:53

Small and Wide Angle X-Ray Scattering Studies of Biological Macromolecules in Solution

Published on: January 8, 2013

Area of Science:

  • Biophysics
  • Structural Biology
  • Analytical Chemistry

Background:

  • Structural analysis of biomolecules is crucial for understanding their function.
  • Traditional methods often require large sample volumes and extensive processing.
  • High-throughput analysis is needed to accelerate biological research.

Purpose of the Study:

  • To present a novel method for automated structural analysis of biomolecules in solution using a microfluidic chip.
  • To develop a micrototal analysis system for high-throughput Small-Angle X-ray Scattering (SAXS) data collection.
  • To validate the system's performance with a model protein system.

Main Methods:

  • Development of a polymer-based microfluidic chip (bioXTAS chip) with an integrated X-ray transparent sample chamber.
  • Implementation of diffusion-based mixing of protein and buffer solutions on-chip.
  • Creation of software for automated fluidic control, data acquisition, and data analysis.
  • Utilizing Small-Angle X-ray Scattering (SAXS) for structural analysis.

Main Results:

  • Demonstrated successful high-throughput SAXS data collection from small sample volumes (200 nL).
  • Validated the on-chip mixing capabilities for buffer exchange and sample preparation.
  • Successfully monitored gradual unfolding of bovine serum albumin (BSA) induced by a surfactant, confirming structural change detection.
  • Achieved automated structural analysis of biomolecules in solution.

Conclusions:

  • The bioXTAS chip enables automated, high-throughput structural analysis of biomolecules in solution.
  • The system is effective for studying structural changes and requires minimal sample volumes.
  • This technology has the potential to become a powerful tool in structural biology and drug discovery.