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Unravelling the Function of a Bacterial Effector from a Non-cultivable Plant Pathogen Using a Yeast Two-hybrid Screen
Published on: January 20, 2017
Yeast as a model host to explore plant virus-host interactions
1Department of Plant Pathology, University of Kentucky, Lexington, Kentucky 40546, USA. pdnagy2@uky.edu
Annual Review of Phytopathology
|April 22, 2008
Summary
Yeast models reveal how plant viruses replicate and evolve by hijacking host cell machinery. Studies identified distinct host factors and host roles in virus evolution, aiding antiviral development.
Area of Science:
- Molecular Biology
- Virology
- Yeast Genetics
Background:
- The yeast Saccharomyces cerevisiae is a model organism for eukaryotic cell processes.
- Plant viruses are obligate intracellular parasites utilizing host cell resources for replication.
Purpose of the Study:
- To use yeast as a model system to study plant virus-host interactions.
- To identify host factors influencing plant virus replication and evolution.
Main Methods:
- Genome-wide and proteomic studies in yeast.
- Investigating bromoviruses and tombusviruses replication in yeast.
Main Results:
- Identified replication-associated factors affecting host-virus interactions, pathology, evolution, and host range.
- Discovered distinct host genes influencing replication for different virus families.
- Found numerous yeast genes impacting tombusvirus RNA-RNA recombination, offering insights into virus evolution.
Conclusions:
- Yeast is a powerful model for dissecting plant virus replication and host interactions.
- Plant viruses employ diverse strategies to exploit host cell resources.
- Host factors play a significant role in viral evolution, particularly RNA-RNA recombination.
- Findings can inform antiviral strategies, biotechnology, and fundamental cell biology research.
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