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Updated: Jul 5, 2026

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus

Published on: September 5, 2013

What is community-associated methicillin-resistant Staphylococcus aureus?

Michael Z David¹, Daniel Glikman, Susan E Crawford

¹Infectious Diseases Section, Department of Pediatrics, the University of Chicago, IL 60637, USA. mdavid@medicine.bsd.uchicago.edu

The Journal of Infectious Diseases

|April 22, 2008

Summary

Healthcare exposure risk factors poorly predict community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) infection characteristics. Defining CA-MRSA solely by absence of risk factors underestimates the true burden of epidemic CA-MRSA disease.

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Last Updated: Jul 5, 2026

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus

Published on: September 5, 2013

Area of Science:

Infectious Diseases
Microbiology
Epidemiology

Background:

Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) is defined by the absence of healthcare exposure risk factors.
This study investigated if this definition accurately predicts CA-MRSA strain characteristics.

Discussion:

Healthcare-associated risk factors had limited predictive value in distinguishing between multidrug-resistant MRSA and CA-MRSA isolates.
A significant proportion of MRSA isolates from patients with healthcare exposure exhibited characteristics typically associated with CA-MRSA.

Key Insights:

Clindamycin susceptibility, Panton-Valentine leukocidin (PVL) gene presence, staphylococcal cassette chromosome mec (SCCmec) type IV, and sequence type 8 (ST8) are key genotypic and phenotypic markers.
Many MRSA isolates from patients with healthcare exposure displayed these CA-MRSA markers.

Outlook:

Rethinking the definition of CA-MRSA is crucial for accurate disease burden assessment.
Epidemiological surveillance and clinical management strategies may need adjustment based on these findings.