Related Experiment Video
Updated: Jul 5, 2026

Microinjection of Medaka Embryos for use as a Model Genetic Organism
Published on: December 22, 2010
Generation of transgenic medaka using modified bacterial artificial chromosome
Shuhei Nakamura1, Daisuke Saito, Minoru Tanaka
1Laboratory of Molecular Genetics for Reproduction, National Institute for Basic Biology, Okazaki, Japan.
Abstract:
The availability of bacterial artificial chromosome (BAC) offers a good genomic platform for a targeted integration of an exogenous gene by a homologous recombination system in Escherichia coli. In combination with microinjection technology, this system allows for the analysis of various aspects of biological phenomena occurring in vivo using Japanese medaka fish (Oryzias latipes). Here we describe a streamlined procedure for selecting BAC clones based on the medaka University of Tokyo genome browser (UTGB), followed by rapid modification with enhanced green fluorescent protein (EGFP) or DsRed fragments for transgenic analysis in medaka. Experimental procedures for BAC DNA preparation, microinjection of medaka embryos and screening of resulting transgenic medaka carrying EGFP/DsRed modified BAC clones are also described.
Related Concept Videos
Bacterial Transformation
Transgenic Organisms
Transgenic Plants
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...

