Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

¹H NMR: Interpreting Distorted and Overlapping Signals01:02

¹H NMR: Interpreting Distorted and Overlapping Signals

Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are slanted or...
NMR Spectroscopy: Chemical Shift Overview01:15

NMR Spectroscopy: Chemical Shift Overview

The position of the absorption signal of a sample is reported relative to the position of the signal of tetramethylsilane (TMS), which is added as an internal reference while recording spectra. The difference between the absorption frequencies of the sample and TMS (in Hz) is divided by the spectrometer operating frequency (in MHz) to obtain a dimensionless quantity called the chemical shift. It is reported on the δ (delta) scale and expressed in parts per million.
For instance, the proton...
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
Proton (¹H) NMR: Chemical Shift01:07

Proton (¹H) NMR: Chemical Shift

Organic molecules primarily contain carbon and hydrogen atoms. While all the hydrogen isotopes are NMR-active, protium or hydrogen-1 is the most abundant. It has a significant energy separation between its nuclear spin states due to its large gyromagnetic ratio. As per Boltzmann's distribution, an increase in the energy separation implies a greater excess population of nuclei available for excitation, resulting in a strong NMR absorption signal.
Absorption signals of all the protium nuclei in a...
2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)01:19

2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)

Heteronuclear single-quantum correlation spectroscopy (HSQC) is a 2D NMR technique that reveals one-bond correlations between hydrogen and a heteronucleus. The HSQC experiment is similar to the heteronuclear correlation experiment (HETCOR) but is more sensitive. In the HSQC spectrum, the proton chemical shift is plotted on the horizontal F2 axis, while the 13C chemical shift is plotted on the vertical F1 axis. The corresponding proton and 13C spectra are also shown. The HSQC contour plot does...
Pharmacokinetics in Pediatric Patients: Drug Metabolism01:24

Pharmacokinetics in Pediatric Patients: Drug Metabolism

In pediatric care, understanding the nuances of hepatic drug metabolism is crucial, as it significantly differs from that of adults. This divergence is primarily due to the developmental stage of drug-metabolizing enzymes, which affects how medications are processed in the body. In neonates, for instance, the activity of Phase I enzymes—critical for the initial breakdown of drugs—is markedly reduced, functioning at just 20–40% of the levels seen in adults. This reduction poses a challenge in...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Liver Fat Fraction and Machine Learning Improve Steatohepatitis Diagnosis in Liver Transplant Patients.

NMR in biomedicine·2025
Same author

Corrigendum to "Comparison of ultrasound to MR and histological methods for liver fat quantification". Eur. J. Radiol. 183 (2025) 111931.

European journal of radiology·2025
Same author

Comparison of ultrasound to MR and histological methods for liver fat quantification.

European journal of radiology·2025
Same author

Skeletal Muscle <sup>31</sup>P MR Spectroscopy Surpasses CT in Predicting Patient Survival After Liver Transplantation.

Journal of cachexia, sarcopenia and muscle·2024
Same author

Brain morphometry in hepatic Wilson disease patients.

Journal of inherited metabolic disease·2024
Same author

Semaglutide Treatment Effects on Liver Fat Content in Obese Subjects with Metabolic-Associated Steatotic Liver Disease (MASLD).

Journal of clinical medicine·2024

Related Experiment Video

Updated: Jul 5, 2026

Quantitative Measure of Lung Structure and Function Obtained from Hyperpolarized Xenon Spectroscopy
08:23

Quantitative Measure of Lung Structure and Function Obtained from Hyperpolarized Xenon Spectroscopy

Published on: November 10, 2023

(1)H MR spectroscopy in pediatrics.

Monika Dezortova1, Milan Hajek1

  • 1MR Unit, Department of Diagnostic and Interventional Radiology, Institute for Clinical and Experimental Medicine, Videnska 1958/9, 140 21 Prague, Czech Republic.

European Journal of Radiology
|April 22, 2008
PubMed
Summary

Brain development impacts metabolite concentrations, crucial for diagnosing pediatric diseases. This study details MR spectroscopy in children, analyzing age-related spectral changes and metabolite alterations in various conditions.

More Related Videos

Use of Electron Paramagnetic Resonance in Biological Samples at Ambient Temperature and 77 K
06:45

Use of Electron Paramagnetic Resonance in Biological Samples at Ambient Temperature and 77 K

Published on: January 11, 2019

How to Administer Near-Infrared Spectroscopy in Critically ill Neonates, Infants, and Children
07:27

How to Administer Near-Infrared Spectroscopy in Critically ill Neonates, Infants, and Children

Published on: August 19, 2020

Related Experiment Videos

Last Updated: Jul 5, 2026

Quantitative Measure of Lung Structure and Function Obtained from Hyperpolarized Xenon Spectroscopy
08:23

Quantitative Measure of Lung Structure and Function Obtained from Hyperpolarized Xenon Spectroscopy

Published on: November 10, 2023

Use of Electron Paramagnetic Resonance in Biological Samples at Ambient Temperature and 77 K
06:45

Use of Electron Paramagnetic Resonance in Biological Samples at Ambient Temperature and 77 K

Published on: January 11, 2019

How to Administer Near-Infrared Spectroscopy in Critically ill Neonates, Infants, and Children
07:27

How to Administer Near-Infrared Spectroscopy in Critically ill Neonates, Infants, and Children

Published on: August 19, 2020

Area of Science:

  • Neuroscience
  • Biochemistry
  • Medical Imaging

Background:

  • Brain development significantly alters metabolite concentrations and spectral appearance.
  • Understanding these changes is vital for identifying pediatric neurological disorders.
  • Magnetic Resonance (MR) spectroscopy is a key tool for non-invasive metabolite assessment.

Purpose of the Study:

  • To discuss specific conditions for MR spectroscopic examination in pediatric patients.
  • To analyze the effects of age on MR spectra quality and metabolite quantitation.
  • To illustrate how diseases manifest as changes in (1)H MR spectra.

Main Methods:

  • Review of pediatric MR spectroscopic examination protocols.
  • Analysis of age-dependent variations in MR spectral data.
  • Correlation of spectral findings with known biochemical alterations in diseases.

Main Results:

  • Age influences MR spectra quality and the accurate measurement of metabolites.
  • Specific metabolite changes in (1)H MR spectra are indicative of various pediatric diseases.
  • Key metabolites like N-acetylaspartate, creatine/phosphocreatine, cholines, lactate, and inositol show significant alterations.

Conclusions:

  • MR spectroscopy is essential for detecting pathological changes in pediatric brain development.
  • Age-specific considerations are critical for reliable MR spectroscopic analysis in children.
  • Altered metabolite profiles in (1)H MR spectra provide insights into pediatric disease pathophysiology.