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Related Experiment Video

Updated: Jul 5, 2026

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus
13:41

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus

Published on: March 8, 2012

Type-specific reproducibility of the Roche linear array HPV genotyping test.

Martin Steinau1, David C Swan, Elizabeth R Unger

  • 1Division of Viral and Rickettsial Diseases, National Center for Zoonotic, Vector-borne and Enteric Diseases, Coordinating Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA. Msteinau@cdc.gov

Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology
|April 22, 2008
PubMed
Summary

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The linear array HPV genotyping test shows good, but not perfect, type-specific reproducibility. This finding is crucial for evaluating HPV typing methods and understanding human papillomavirus prevalence for vaccination programs.

Area of Science:

  • Virology
  • Genetics
  • Public Health

Background:

  • Type-specific human papillomavirus (HPV) detection is vital for monitoring prevalence changes to support HPV vaccination strategies.
  • Understanding the influence of sampling, extraction, and assay characteristics on HPV test results is increasingly recognized.
  • While inter-assay comparability has been studied, the robustness and intra-assay reproducibility of type-specific HPV results require further emphasis.

Purpose of the Study:

  • To assess the general and type-specific reproducibility of the linear array HPV genotyping test.
  • To evaluate the reliability of a specific HPV typing method for epidemiological studies and vaccination efforts.

Main Methods:

  • Retesting of 276 cervical samples from two ongoing HPV epidemiological studies under blinded conditions.

Related Experiment Videos

Last Updated: Jul 5, 2026

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus
13:41

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus

Published on: March 8, 2012

  • Utilizing five different reagent lots and three technologists to assess reproducibility across various conditions.
  • Statistical analysis including concordance rates and kappa statistics to quantify agreement.

    • Main Results:

    • High overall concordance for HPV detection (98.2%, kappa=0.959) across all 37 types.
    • Excellent type-specific concordance for individual HPV types (99.4%, kappa=0.915).
    • Complete concordance for all types observed in 83.0% of the samples tested.

    Conclusions:

    • The linear array HPV genotyping test demonstrates good, though not absolute, type-specific reproducibility.
    • The findings underscore the importance of including type-specific performance metrics when evaluating HPV typing assays.
    • Results support the use of this test in epidemiological surveillance for HPV, contributing to informed vaccination strategies.