Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

T Cell Types and Functions01:24

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
Inflammatory Bowel Disease III: Crohn's Disease01:25

Inflammatory Bowel Disease III: Crohn's Disease

Crohn’s disease is a chronic, relapsing form of inflammatory bowel disease characterized by segmental, transmural inflammation that can affect any part of the gastrointestinal tract. Its pathogenesis arises from a combination of genetic susceptibility, environmental exposures, epithelial barrier dysfunction, and immune dysregulation. Together, these factors lead to an exaggerated immune response against components of the gut microbiome.Genetic and Environmental InfluencesMultiple genetic...
T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
Inflammatory Response01:28

Inflammatory Response

An inflammatory response is a localized, nonspecific immune reaction that occurs when a tissue is injured. It is characterized by redness, swelling, heat, and pain, which are commonly called the cardinal signs and symptoms of inflammation. Inflammation can sometimes result in a loss of function.
Inflammation can be triggered by various stimuli, such as impact, abrasion, chemical irritation, infections, and extreme hot or cold temperatures. These can damage cells and connective tissue fibers,...
B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evaluation of neutrophil oxidative burst capacity in relation to major adverse cardiac events and infection in patients with cardiovascular disease.

Clinical research in cardiology : official journal of the German Cardiac Society·2026
Same author

Antibody S22019F Selectively Recognises KIR2DS1 and Enables Analysis of KIR2DS1<sup>+</sup> NK Cells and T Cells.

HLA·2026
Same author

Soluble CD83 Accelerates Wound Healing and Attenuates Inflammatory Responses Induced by Chronic Wound Fluid in a Human 3D in Vitro Wound Healing Model.

Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society·2026
Same author

Measuring the Degree of Labeling of Antibody-Dye Conjugates with a Single-Molecule-Sensitive Digital Flow Cytometer.

Analytical chemistry·2026
Same author

Thyroid hormone receptor α signaling shapes innate and adaptive immune responses during viral infection.

European thyroid journal·2025
Same author

Influence of amitriptyline therapy on T cell phenotype in chronic pain patients.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2025

Related Experiment Video

Updated: Jul 5, 2026

Induction of Murine Intestinal Inflammation by Adoptive Transfer of Effector CD4+CD45RBhigh T Cells into Immunodeficient Mice
08:37

Induction of Murine Intestinal Inflammation by Adoptive Transfer of Effector CD4+CD45RBhigh T Cells into Immunodeficient Mice

Published on: April 21, 2015

CD83 expression in CD4+ T cells modulates inflammation and autoimmunity.

Simone Reinwald1, Carsten Wiethe, Astrid M Westendorf

  • 1Department of Mucosal Immunity, Helmholtz Centre for Infection Research, Braunschweig, Germany.

Journal of Immunology (Baltimore, Md. : 1950)
|April 22, 2008
PubMed
Summary

CD83 protein expression on CD4(+) T cells confers immunosuppressive functions. This study demonstrates CD83

More Related Videos

Isolation and Th17 Differentiation of Na&iuml;ve CD4 T Lymphocytes
12:59

Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes

Published on: September 26, 2013

Mouse Na&#239;ve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets
07:12

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets

Published on: April 16, 2015

Related Experiment Videos

Last Updated: Jul 5, 2026

Induction of Murine Intestinal Inflammation by Adoptive Transfer of Effector CD4+CD45RBhigh T Cells into Immunodeficient Mice
08:37

Induction of Murine Intestinal Inflammation by Adoptive Transfer of Effector CD4+CD45RBhigh T Cells into Immunodeficient Mice

Published on: April 21, 2015

Isolation and Th17 Differentiation of Na&iuml;ve CD4 T Lymphocytes
12:59

Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes

Published on: September 26, 2013

Mouse Na&#239;ve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets
07:12

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets

Published on: April 16, 2015

Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Biology

Background:

  • CD83, a transmembrane protein, is known as a dendritic cell maturation marker.
  • Emerging evidence suggests CD83 also influences B cell function, T cell maturation, and T cell activation.
  • The role of CD83 in T cell-mediated immunosuppression remains largely unexplored.

Purpose of the Study:

  • To investigate the role of CD83 expression in the immunosuppressive function of CD4(+) T cells.
  • To determine if CD83 can induce a regulatory T cell phenotype.

Main Methods:

  • Analysis of CD83 mRNA expression in regulatory T cells (Tregs).
  • Retroviral transduction of naive CD4(+)CD25(-) T cells with CD83.
  • In vitro induction of regulatory phenotype and Foxp3 expression.
  • In vivo functional assays including contact hypersensitivity and experimental autoimmune encephalomyelitis (EAE) models.

Main Results:

  • CD83 mRNA is differentially expressed in natural CD4(+)CD25(+) regulatory T cells.
  • Activated T cells rapidly express surface CD83.
  • CD83 transduction into naive T cells induces a regulatory phenotype, including Foxp3 induction.
  • CD83(+)Foxp3(+) T cells suppressed skin contact hypersensitivity reactions in vivo.
  • Adoptive transfer of CD83-expressing T cells ameliorated EAE, reduced pro-inflammatory cytokines (IFN-γ, IL-17), and increased IL-10.

Conclusions:

  • CD83 expression confers immunosuppressive properties to CD4(+) T cells.
  • CD83 can induce a regulatory T cell phenotype in vitro and in vivo.
  • These findings establish CD83 as a novel factor contributing to CD4(+) T cell-mediated immunosuppression.