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Updated: Jul 5, 2026

A New Approach for the Comparative Analysis of Multiprotein Complexes Based on 15N Metabolic Labeling and Quantitative Mass Spectrometry
Published on: March 13, 2014
Isotopic labelling of photosystem II in Thermosynechococcus elongatus
Alain Boussac1, Jean-Marc Verbavatz, Miwa Sugiura
1iBiTec-S, URA CNRS 2096, CEA Saclay, 91191, Gif sur Yvette, France. alain.boussac@cea.fr
Abstract:
This report describes a protocol to incorporate isotopically labelled aromatic amino acids into the proteins of the thermophilic cyanobacterium Thermosynechoccus elongatus. By using the EPR signal of the two redox active tyrosines of Photosystem II, Tyr(D)(*) and Tyr(Z)(*), as spectroscopic probes it is shown that labelled tyrosines can be incorporated with a high yield in this cyanobacterium. The production of a fully (13)C- or (2)H-labelled enzyme is also described.
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