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Updated: Jul 5, 2026

Two Peeling Methods for the Isolation of Photoreceptor Cell Compartments in the Mouse Retina for Protein Analysis
Published on: December 7, 2021
Light-dependent compartmentalization of transducin in rod photoreceptors
1Department of Molecular Physiology and Biophysics, University of Iowa College of Medicine, 5-532 Bowen Science Building, 51 Newton Road, Iowa, IA 52242, USA. nikolai-artemyev@uiowa.edu
Abstract:
Three major visual signaling proteins, transducin, arrestin, and recoverin undergo bidirectional translocations between the outer segment and inner compartments of rod photoreceptors in a light-dependent manner. The light-dependent translocation of proteins is believed to contribute to adaptation and neuroprotection of photoreceptor cells. The potential physiological significance and mechanisms of light-controlled protein translocations are at the center of current discussion. In this paper, I outline the latest advances in understanding the mechanisms of bidirectional translocation of transducin and determinants of its steady-state distribution in dark- and light-adapted photoreceptor cells.
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