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Updated: Jul 5, 2026

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
Published on: October 7, 2025
In situ polymerase chain reaction and hybridization to detect low-abundance nucleic acid targets
1South Carolina Center for Biotechnology, Claflin University, Orangeburg, South Carolina, USA.
Abstract:
This unit provides detailed methods and material descriptions for in situ hybridization following in situ amplification of DNA or RNA by PCR. It includes all essential components of the techniques, including variations suitable for different kinds of tissue and cell preparations. Planning, controls, and critical parameters for the amplification steps are discussed.
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